新型异黄酮及甾体类化合物抗辐射作用的实验研究  

Research on the irradiation protection of isoflavone and steroid derivatives

作　　者：汤洁[1] 粟永萍[2] 艾国平[2] 张丽龙[2] 王涛[2] 程天民[2] 

机构地区：[1]安徽医科大学附属省立医院老年心血管科,安徽省心血管病研究所,安徽合肥230001 [2]第三军医大学军事预防医学院防原医学教研室,全军复合伤研究所,创伤,烧伤与复合伤国家重点实验室,重庆400038

出　　处：《安徽医药》2009年第12期1474-1477,共4页Anhui Medical and Pharmaceutical Journal

基　　金：重庆市院士基金(No2004BC5006)

摘　　要：目的对进行改构的两种异黄酮类化合物F12、F2及一种甾体类化合物ZE1进行体外、体内实验,寻找一种新型的抗辐射药物。方法以Genistein和雌二醇为阳性对照,应用MTT法对比研究三种化合物在不同的浓度对IEC-6、巨核细胞系-Dami细胞株细胞增殖活性的影响;并观察其对8Gy和12Gy60Co-γ射线照射小鼠肠道及造血辐射防护效果。结果F2、F12、ZE1在不同浓度对IEC-6及Dami细胞均有不同的辐射防护作用,效果显著优于Genistein和雌二醇(P<0.01)。动物实验结果显示F2使辐射损伤小鼠的骨髓和脾脏有核细胞数量增加,肠上皮细胞绒毛高度增加,隐窝变长,效果与雌二醇相当,而未观察到明显的毒副反应。结论经过特定结构修饰的异黄酮类化合物F2,有较好的辐射保护作用,同时无明显的雌激素样作用,提示可能是较为理想的辐射防护剂候选药物。Aim Assumed to modify the structure of isoflavone and steroid derivatives compounds F12, F2,ZE1 and analyze them so as to obtain a new radioprotective drug with low toxicity. Methods Adding the compounds into IEC-6 and Dami cell lines, then irradiated with ^60CO-γ rays. measuring the cell＇ s survival rate by MTT; mice were treated with radiation and compounds to investigate the hematology and intestinal irradiation protective effects of the compounds. Results F12 and F2 can promote the irradiated IEC-6 and Dami cell＇ s proliferation evidently compared with the radiation group both before and after irradiating is effective. Conclusion F2 can protect the intestinal of irradiated mice, and has low toxicity to vitals, it probably will be a perfect radioprotective drug.

关 键 词：异黄酮 甾体 造血功能 辐射防护 MTT 

分 类 号：R285[医药卫生—中药学] R282.77[医药卫生—中医学]