全国不同产地白英总生物碱的含量测定  

Determination of the Total Alkaloid Content of Solanum lyratum of Different Places of Production

作　　者：李晨[1] 卫莹芳[2] 许一江[2] 刘永[2] 谢达温[2] 

机构地区：[1]成都医学院药学院,四川成都610038 [2]成都中医药大学,四川成都610075

出　　处：《四川中医》2009年第12期65-66,共2页Journal of Sichuan of Traditional Chinese Medicine

摘　　要：目的:建立白英总生物碱含量测定方法。方法:紫外-可见分光光度法,以澳洲茄胺为对照品,测定波长为414nm。结果:澳洲茄胺在0.016mg～0.096mg范围内有良好的线性关系,计算得回归方程:Y=0.1466X+0.133,R=0.9998;加样回收率为100.05%(RSD=1.281%);供试品溶液在0～2h内稳定(RSD=0.354%)。全国不同产地白英药材26个样品中甾体总生物碱的含量在0.08%～0.24%之间。结论:该方法简便、快速、测定结果准确,可用于白英总生物碱的含量测定。Objectives：Establishing a method of determining the total alkaloid content of Solanum lyratum.Methods：Solasodine serves for contrasting;the contents are mensurated by UV at the wavelength of 414nm.Results：Solasodine shows a good linear relationship at the range of 0.016mg～0.096mg.Calculate the regressiveequation：Y=0.1466X±0.133,R2=0.9998;The mean recovery is 100.05%（RSD=1.281%）,the sample solution is steady within 2 hours（RSD=0.354%）.The steroid total alkaloid contents of 26 Solanum lyratum samples which are from different places of production in China are between 0.08% - 0.24% almost. Conclusion： It is a easy method to mensurate the total alkaloid content of Solarium lyratum and can obtain the accurate results. It also can provide the reference to establish the quality standard of Solanum lyratum.

关 键 词：白英 总生物碱 紫外-可见分分光光度法 

分 类 号：R284.2[医药卫生—中药学]