HPLC-ELSD法测定三维肌醇片中肌醇的含量  

Content Determination of Inositol in Trivitamins and Inositol Tablets by HPLC-ELSD

作　　者：魏钰茜[1] 刘倩[2] 王丽[3] 赵仁永 

机构地区：[1]解放军总医院南楼药局,北京100853 [2]北京市肿瘤医院,北京100036 [3]山东赛克赛斯药业科技有限公司,济南250101 [4]山东省医药工业研究所,济南250100

出　　处：《中国药物应用与监测》2009年第6期341-343,共3页Chinese Journal of Drug Application and Monitoring

摘　　要：目的:建立简便准确的测定三维肌醇片中肌醇含量的方法。方法:采用HPLC-ELSD方法进行测定,色谱柱为UltimateTMXB-NH(24.6mm×250mm,5μm),流动相为乙腈-水(70∶30),流速0.8mL.min-1,漂移管温度100℃,雾化气流速度2.5L.min-1。结果:肌醇在0.1016～1.016mg.mL-1范围内线性关系良好,r=0.9997,平均回收率102.32%,RSD=1.41%,样品中其他成分不干扰测定。结论:本方法简便可靠,可用于复方制剂中肌醇含量的质量控制。Objective： To establish a simple and accurate method for the content determination of inositol in trivitamins and inositol tablets. Methods： The assay was performed by HPLC-ELSD method. An UltimateTM XB-NH2 column （4.6 mm × 250 mm, 5 μm） was used for separation. Acetonitrile-water （70 ： 30） was used as mobile phase at a flow rate of 0.8 mL·min^-1. The temperature of drift tube was 100 ℃, and the speed of atomizing flow was 2.5 L·min^-1. Results： Under the above described chromatographic conditions, the linear ranges of inositol was 0.101 6 - 1.016 mg· mL^-1 （r = 0.999 7）, and the mean recovery rate was 102.32% （RSD = 1.41%）. The other ingredients in the sample showed no interference for the determination of inositol. Conclusion： The assay method is simple and accurate for the quality control of inositol in compound preparations.

关 键 词：肌醇 高效液相色谱蒸发光检测 含量测定 

分 类 号：R927.2[医药卫生—药学]