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机构地区:[1]黑龙江八一农垦大学,大庆163319 [2]大庆市银浪牧场,大庆163412
出 处:《动物营养学报》2009年第6期967-973,共7页CHINESE JOURNAL OF ANIMAL NUTRITION
基 金:黑龙江农垦总局科技攻关项目(HNKXV-08-04A)
摘 要:为了探讨半胱胺和大豆黄酮对东北细毛羊肝脏、肌肉和皮肤组织中IGF-ⅠmRNA表达量的影响,选取体重27~28 kg,5~6月龄健康的东北细毛羊育成羊21只,随机分成7组,每组3个重复,每个重复1只,采用实时荧光定量PCR法检测肝脏、肌肉和皮肤组织中IGF-ⅠmRNA相对表达量。结果表明:(1)半胱胺和大豆黄酮均能显著提高东北细毛羊的生长性能(P<0.05),其中以7.5 mg/kg BW半胱胺组和7.5 mg/kg BW半胱胺+3 mg/kg BW大豆黄酮组效果较佳;(2)半胱胺和大豆黄酮对东北细毛羊肝脏和肌肉中IGF-ⅠmRNA表达量有显著的提高作用(P<0.05),但各试验组之间差异不显著(P>0.05);(3)对皮肤中表达量的影响:对照组与添加3和6 mg/kg BW大豆黄酮组和7.5 mg/kg BW半胱胺+3 mg/kg BW大豆黄酮组之间差异显著(P<0.05),与添加7.5和15 mg/kg BW半胱胺组和15 mg/kg BW半胱胺+6 mg/kg BW大豆黄酮组之间差异不显著(P>0.05)。由结果可知,半胱胺和大豆黄酮均能提高肝脏和肌肉中IGF-ⅠmRNA的表达量,但协同应用并没有促使IGF-ⅠmRNA表达量在肝脏、肌肉和皮肤组织中的明显提高,综合分析以添加3 mg/kg BW大豆黄酮效果最佳。In order to explore the effects of cysteamine and daidzein on gene expression of IGF- Ⅰ mRNA in the tissue of liver, muscle and skin in Northeast fine-wool sheep. Twenty-one healthy Northeast fine-wool sheep with body weight of 27- 28 kg and age of 5--6-month-old were randomly divided into 7 groups with 3 replicates and each replicate had 1 sheep. Using real-time fluorescence quantitative PCR detected the IGF-Ⅰ mRNA expression on liver, muscle and skin. The results showed as follows: 1) both cysteamine and daidzein could significantly enhance the growth performance of Northeast fine- wool sheep (P〈0.05), and the 7.5 mg/kg BW cysteamine group and 7.5 mg/kg BW cysteamine + 3 mg/kg BW daidzein group had the better effects; 2) cysteamine and daidzein could evidently increased the expression of gene IGF- Ⅰ in the liver and muscle of Northeast fine-wool sheep (P〈0.05), but no significant difference was found among trial groups (P〉0.05) ; 3) compared with the control group, the IGF- Ⅰ mRNA expression on the skin in 3 mg/kg BW daidzein group, 6 mg/kg BW daidzein group and 7.5 mg/kg BW cysteamine + 3 mg/kg BW daidzein group were significantly improved (P〈0.05), but no significant difference was found among the control group, 7.5 mg/kg BW cysteamine group, 15 mg/kg BW cysteamine group and 15 mg/kg BW cysteamine + 6 mg/kg BW daidzein group (P〉0.05). It was concluded that cysteamine and daidzein could improve the IGF- Ⅰ mRNA expression in liver and muscles, but cooperation application did not make the gene IGF- Ⅰ mRNA expression obviously improved in the liver, muscle and skin tissue. In summary, adding 3 mg/kg BW of daidzein had the best effect.
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