哺乳仔猪空肠FBX032基因表达及肠上皮形态结构的分析  

Study on FBXO32 Gene Expression and Enteric Epithelium Structure of Jejunum in Suckling Piglets

作　　者：孙云子[1,2] 余冰[1,3] 张克英[1] 何军[1,3] 陈代文[1,3] 

机构地区：[1]四川农业大学动物营养研究所,雅安625014 [2]贵州省六盘水市畜牧局,六盘水561600 [3]教育部动物抗病营养重点实验室,雅安625014

出　　处：《动物营养学报》2009年第6期987-992,共6页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：教育部创新团队猪抗病营养分子机制项目(编号:IRTO555-5);四川省教育厅重点项目:仔猪肠道功能基因表达与营养调控;贵州省农业厅及贵州六盘水市科技局项目

摘　　要：本研究旨在探讨哺乳仔猪空肠FBXO32基因表达以及空肠上皮细胞形态组织结构的变化。分别采集出生当天(0日龄)、3、8、14和21日龄5个时间点的大约克哺乳仔猪空肠样品作为研究对象,采用实时荧光定量PCR检测FBXO32基因的相对表达量,并在光镜和透射电镜下观察肠上皮细胞形态结构的变化。结果表明,在哺乳仔猪空肠有FBXO32基因表达,与0日龄相比,FBXO32基因在3、14和21日龄的表达水平上调,上调幅度分别为1.16、3.89和11.83倍,在8日龄表达下调,下调为0日龄的53%;光学显微镜观察结果表明,和0日龄相比,哺乳仔猪肠绒毛个数、高度、宽度和隐窝深度显著增加(P<0.05或P<0.01)。透射电镜观察显示,哺乳阶段的仔猪空肠上皮细胞超微结构普遍具有微绒毛整齐度很好,游离核糖体、线粒体和粗面内质网丰富的特征,在3和8日龄时出现部分线粒体轻微肿胀,而在14和21日龄时未见这种现象。以上结果表明哺乳仔猪空肠FBXO32基因表达及形态结构均有显著变化。The experiment was conducted to examine the expression of FBXO32 and the epithelium structure of jejunum in the suckling piglets. Real-time quantitative reverse transcription PCR was carried out to quantify FBXO32 expression on day 3, 8, 14 and 21 and the day on birth （day 0） for Yorkshire piglets. At the same time the epithelium structure of jejunum was observed by using optical microscope and transmission electron microscope. Results showed that FBXO32 expressed in postnatal jejunum of piglets during suckling. The expression level increased 1.16, 3.89 and 11.83 times on day 3, 14 and 21 compared with that on day 0. The expression level on day 8 decreased to 53% of that on day 0. Optical microscopy results indicated that the number, height and width of jejunum villus and crypt depth significantly increased （P〈0.05 or P〈0.01）. Observation on ultrastructure demonstrated that suckling kept the jejuna microvillus in good uniformity and free ribosomes, mitochondria and rough endoplasmic reticulum in abundance. But mitochondria became slight swelling on day 3 and 8, while there was not such phenomenon on day 14 and 21. In conclusion, the results showed that FBXO32 gene expression and morphology had significantly changed in jejunum of suckling piglets.

关 键 词：仔猪 空肠 FBXO32 肠上皮结构 

分 类 号：S646.15[农业科学—蔬菜学] S828.45[农业科学—园艺学]