RP-HPLC法同时测定雪胆胃肠丸中木香烃内酯和去氢木香内酯的含量  

Simultaneous Determination of the Contents of Costunolide and Dehydrocostuslactone in Xuedan Weichang Pill by RP-HPLC

作　　者：郭兆荣[1,2] 杨世林[1,2,3] 李笑然[1] 郝丽莉[1] 许琼明[1] 陈韶华[1] 

机构地区：[1]苏州大学药学院,苏州市215123 [2]江西中医学院,南昌市330004 [3]中药固体制剂制造技术国家工程技术研究中心,南昌市330006

出　　处：《中国药房》2009年第36期2832-2834,共3页China Pharmacy

基　　金：国家科技支撑计划子课题(2006BA108B03-05)

摘　　要：目的:建立以反相高效液相色谱法同时测定雪胆胃肠丸中木香烃内酯和去氢木香内酯含量的方法。方法:色谱柱为Shim-pack VP-ODS(150mm×4.6mm,5μm),流动相为甲醇-水(60∶40),检测波长为225nm。结果:木香烃内酯、去氢木香内酯的检测浓度分别在13.6～272.0、11.25～225.0μg·mL-1范围内与各自峰面积积分值呈良好的线性关系(r均为0.9998);二者平均回收率分别为99.47%和99.62%,RSD分别为1.75%和1.14%(n均为6)。结论:本方法简便易行、准确可靠,可用于雪胆胃肠丸的质量控制。OBJECTIVE ： To establish an RP - HPLC method for simultaneous determination of the contents of costunolide and dehydrocostuslactone in Xuedan weichang pill. METHODS： The determination was performed on Shim- pack VP- ODS column（150 mm × 4.6 mm, 5 μm） with methanol - water（60 ： 40） as mobile phase and the detection wavelength was set at 225 nm.RESULTS： The linear ranges of costunolide and dehydrocostuslactone were 13.6-272.0 μg·mL^-1 and 11.25-225.0 μg·mL^-1, respectively, and their average recoveries were 99.47% and 99.62%, respectively with RSD of 1.75% （ n = 6） and 1.14% （n = 6）, respectively. CONCLUSION： The method established is simple, feasible, accurate and reliable, and it is applicable for the quality control of Xuedan weichang pill.

关 键 词：雪胆胃肠丸 木香烃内酯 去氢木香内酯 反相高效液相色谱法 含量测定 

分 类 号：R283.64[医药卫生—中药学] R927.2[医药卫生—中医学]