地黄寡糖质量标准研究  

Quality Standard of Dihuang Guatang

作　　者：刘影[1] 杨菁[1] 安阳 刘涛[1] 韦忠民[1] 

机构地区：[1]辽宁医学院细胞生物学及新药开发重点试验室,锦州市121001 [2]锦州奥鸿药业有限责任公司,锦州市121000

出　　处：《中国药房》2009年第36期2834-2835,共2页China Pharmacy

基　　金：辽宁省博士启动;自然科学基金项目(20072202);辽宁省教育厅2007~2008年度创新团队项目计划(2008T115)

摘　　要：目的:建立地黄寡糖的质量标准。方法:采用薄层色谱(TLC)法对方中的水苏糖进行定性鉴别;以高效液相色谱法测定梓醇及高分子物质的含量,干燥失重法测定干物质含量,苯酚-硫酸法测定寡糖含量。结果:TLC斑点清晰,分离度好;梓醇进样量在5～80μg(r=0.9975)范围内与峰面积积分值呈良好线性关系,平均回收率为98.5%,RSD=1.28%(n=6);高分子物质的含量≤2.0%;干物质含量为18.0～22.0g·L-1;寡糖含量均>85.0%。结论:所建标准可用于地黄寡糖的质量控制。OBJECTIVE： To establish the quality standard of Dihuang guatang. METHODS： The quality of stachyose in Dihuang guatang was identified by TLC.The contents of catalpol and high- molecular substance were determined by HPLC.The content of dry substance was determined by drying method. The content of oligosaceharides was determined by phenol- sulfuric acid method. RESULTS： The TLC spots of stachyose were distinctive and well- isolated. The linear relationship of catalpol was good in the range of 5-80μg（ r = 0.997 5） with an average recovery of 98.5% （RSD = 1.28%, n = 6）. The content of high- molecular substances was less than 2.0% .The content of dry substance was 18.0-22.0 g· L^- 1.The content of oligosaccharides was more than 85.0%. CONCLUSION： The established quality standard can be used for the quality control of Dihuang guatang.

关 键 词：地黄寡糖 质量标准 薄层色谱法 高效液相色谱法 干燥失重法 苯酚-硫酸法 

分 类 号：R284.2[医药卫生—中药学] R927.1[医药卫生—中医学]