水稻OsWTF1基因启动子的克隆与表达分析  

Cloning and Expression Properties of Rice OsWTF1 Gene Promoter

作　　者：李尉[1] 周小云[1] 王育华[1] 许发喜[1] 陈信波[1] 

机构地区：[1]湖南农业大学国家重点实验室培育基地作物基因工程实验室,湖南长沙410128

出　　处：《现代生物医学进展》2009年第20期3816-3819,共4页Progress in Modern Biomedicine

基　　金：国家973计划前期研究专项(2007CB116207)

摘　　要：目的:分析水稻OsWTF1基因启动子的功能及核心序列。方法:利用PCR技术从水稻日本晴基因组中克隆了转录因子WTF1编码区5'上游大小为2049bp的调控区域,命名为OsWTF1,将它和长度为1631、608、474、415bp的5'端缺失体分别与GUS基因融合构建表达载体,并用农杆菌介导法转化水稻。结果:GUS组织化学分析表明,OsWTF1、Os1631能够驱动GUS基因在根、茎、叶、叶鞘、花药、颖壳上的表达,Os608,Os474,Os415能驱动GUS在根、茎、花药、颖壳中表达,在叶鞘中未表达,而且在叶中的表达也很微弱。结论:OsWTF1启动子核心序列可能位于-1bp--415bp之间,在-608bp--1631bp之间可能存在与基因叶肉特异表达相关的重要元件。Objective： To analyze the function and core fragment of rice OsWTF1 gene promoter. Methods： A 2049 bp promoter fragment office transcription factor WTF1 gene was amplified by PCR from rice（Oryza sativa L. subsp.japonica） and named as OsWTF1. The full length of OsWTF1 and its 5＇ end deletions, the length of which were 1631, 608,474, 415 bp, respectively, were fused with GUS gene. All of the constructed vectors were transformed into rice calli by Agrobacterium-mediated method. Results： The results of histochemical GUS staining showed： the 2049 bp and 1631 bp upstream region of OsWTF1 gene directed GUS expression in roots, stems, leaves, leaf sheath, anther and hull. The GUS gene directed by 608 bp, 474 bp, and 415 bp upstream fragment expressed in roots, stems, anther and hull. But the GUS activity was not detected in leaf sheath, and also weak in leaves. Conclusion： The core sequences of rice OsWTF1 gene promoter may be located in -1 bp- -415 bp, and there may be some leaf-specific cis-acting elements in -608 bp- -1631 bp.

关 键 词：水稻 OsWTF1基因 启动子 

分 类 号：Q943.2[生物学—植物学]