PDGF介导ROCK对血管平滑肌细胞基质金属蛋白酶-2的表达及活性的影响  

Effects of ROCK on the activity of matrix metalloproteinases 2(MMP-2) of vascular smooth muscle cells induced by PDGF

作　　者：洪艳[1] 郭艳[1] 赵莹[2] 崔颖[2] 魏晓晴[2] 吕广艳[2] 陈海波[2] 高颖[1,2] 

机构地区：[1]大连医科大学生化教研室,辽宁大连116044 [2]辽宁省医学细胞分子生物学重点实验室,辽宁大连116044

出　　处：《现代生物医学进展》2009年第20期3855-3857,共3页Progress in Modern Biomedicine

基　　金：辽宁省教育厅科技项目(20060196);辽宁省科技厅自然基金项目(20072166)

摘　　要：目的:研究PDGF介导ROCK亚型(ROCKⅠ和ROCKⅡ)对大鼠胸主动脉平滑肌细胞(A7r5)基质金属蛋白酶2(MMP-2)表达及活性的影响。方法:利用RNA干扰技术使ROCKⅠ,ROCKⅡ基因表达下调,并检测基因下调后蛋白表达水平;使用免疫印迹法(western blot)检测MMP-2蛋白的表达;使用明胶酶谱法检测MMP-2蛋白的活性。结果:通过对A7r5细胞进行ROCKⅠ和ROCKⅡsiRNA转染,二者蛋白表达水平分别下调79.8%和70.1%;ROCKⅠ,ROCKⅡ蛋白表达下调抑制了MMP2的表达和活性,但是ROCKⅡ作用更明显。结论:ROCKⅠ和ROCKⅡ均抑制MMP-2的表达和活性。Objective：To investigate the effects of ROCKⅠ and ROCKⅡ on the expression and activity of matrix metallopr-oteinases 2（MMP-2） of vascular smooth muscle cells（VSMC） induced by PDGF-BB.Methods：ROCKⅠ and ROCKⅡ genes were down-regulated by siRNA transfection.The expression levels of ROCKI/II and MMP-2 were detected by western blot,and the activity of MMP-2 was assessed by Gelatin Zymognaphy.Results：By siRNA transfection,the protein expressions of ROCKⅠ and ROCKⅡ were down regulated by 79.8% and 70.1% respectively; The expression and activity of MMP-2 were inhibited by the down-regulated ROCKⅠ and ROCK Ⅱ, whereas the effect of ROCK Ⅱ was more marked. Conclusion： Both ROCK Ⅰ and ROCK Ⅱ siRNA decreased the expression and activity of MMP-2.

关 键 词：ROCKⅠ ROCKⅡ 血管平滑肌细胞 细胞迁移 基质金属蛋白酶 

分 类 号：Q593[生物学—生物化学] Q244