RECK及MMP-9蛋白在皮肤鳞状细胞癌中的表达及临床病理意义(英文)  

Expression of RECK and MMP-9 in Cutaneous Squamous Cell Carcinoma and its pathological significance

作　　者：亓麟[1] 陈振雨[1] 冷向锋[1] 王彦进[1] 朱月华[1] 

机构地区：[1]青岛大学医学院附属医院整形美容科,山东青岛266000

出　　处：《现代生物医学进展》2009年第20期3903-3906,共4页Progress in Modern Biomedicine

摘　　要：目的:观察RECK基因及基质金属蛋白酶-9(MMP-9)在皮肤鳞状细胞癌(CSCC)中的表达,探讨其与肿瘤浸润转移的关系。方法:应用免疫组化SP法检测40例CSCC组织、17例癌旁不典型增生组织及14例正常皮肤组织中RECK及MMP-9蛋白的表达。结果:①RECK在CSCC、癌旁不典型增生及正常皮肤中的表达率依次增高(30.0%、47.1%、85.7%),组间比较差异有统计学意义(P<0.05);MMP-9蛋白在CSCC、癌旁不典型增生及正常皮肤中的表达率依次降低(82.5%、76.4%、28.6%),组间比较差异有统计学意义(P<0.05)②RECK及MMP-9与CSCC的组织学分级、淋巴结转移密切相关(P均<0.05);与CSCC患者的性别、年龄无关(P均>0.05)③RECK及MMP-9在CSCC中的表达呈负相关(r=-0.475p<0.01)。结论:RECK和MMP-9与CSCC的浸润转移密切相关,RECK在CSCC中表达减少或缺失可能是通过上调MMP-9的表达从而促进CSCC的侵袭与转移。Objective： To investigate the expression of RECK and matrix metaUoproteinase-9 （MMP-9） protein in cutaneous squamous cell carcinoma （CSCC）, and analysis it relationship with the occurrence, development, invasion and metastasis of cancer. Methods： SP immunohistochemical method was used to detect the expression of RECK and MMP-9 protein in 40 cases of patients with cutaneous squamous cell carcinoma, 17 cases of adjacent atypical hyperplasia （AH） and 14 cases of normal cutaneous epithelium （CE）. Results： （~The expression rate of RECK protein increased gradually in CSCC, AH and NE （30.0%, 47.1%, 85.7%, respectively）, and there was significant difference in three groups （P〈0.05）. However, the expression rate of MMP-9 protein decreased gradually in CSCC, AH and CE （82.5%, 76.4 %, 28.6 % respectively） and their difference in three groups has the statistic significance.（P〈0.05）. （~） Both RECK and MMP-9 expression in CSCC was associated with with the tumor grade infiltration and lymph node metastasis. The difference was significant.（P〈0.05）. @RECK expression had negative correlation with MMP-9 expression （ r = -0.475, P〈0.01）. Conclusion： RECK and MMP-9 may play important roles in the invasion and metastasis of CSCC. The down-regulation or loss of RECK expression stimulates migration and metastasis of CSCC by up-regulating MMP-9 expression.

关 键 词：基质金属蛋白酶 MMP-9 鳞状细胞癌 皮肤组织 临床病理 免疫组化SP法 英文 肿瘤浸润 

分 类 号：Q55[生物学—生物化学] Q492.6