慢性间歇性低压低氧对大鼠免疫功能的影响  

EFFECTS OF CHRONIC INTERMITTENT HYPOBARIC HYPOXIA ON IMMUNE FUNCTION IN RAT

作　　者：史敏[1] 崔芳[2] 杨长瑛[2] 张浩[2] 王莹萍[2] 魏林[3] 周兆年[4] 张翼[2] 

机构地区：[1]河北医科大学第二医院检验科,河北石家庄050017 [2]河北医科大学生理学教研室,河北石家庄050017 [3]河北医科大学免疫学教研室,河北石家庄050017 [4]中国科学院上海生命科学研究院,上海200031

出　　处：《中国应用生理学杂志》2009年第4期433-438,共6页Chinese Journal of Applied Physiology

基　　金：国家基础973项目(2006CB504106);国家自然科学基金课题(30572086);国家高校博士点专项基金(20060089009);河北省卫生厅医学科学重点研究课题(05084);河北省科技攻关项目(062761309)

摘　　要：目的:本研究应用流式细胞术、免疫组化和电镜等方法探讨慢性间歇性低压低氧(CIHH)对细胞免疫和体液免疫的影响。方法:雄性成年大鼠48只,随机分为4组:对照组(CON),CIHH14 d组(CIHH14)、CIHH28 d组(CIHH28)和CIHH42 d组(CIHH42)。CIHH动物于低压氧舱分别接受14、28和42 d减压低氧(模拟3 000m高原、每天5 h)处理。各组动物分为两部分,分别在常氧和急性低氧(10%O2+90%N2,1 h)条件下,检测动物血液CD3、CD4、CD8淋巴细胞亚群、NK细胞数量,IgG、糖皮质激素(COR)、肾上腺素(EPI)和C-反应蛋白(CRP)含量,测量胸腺、脾脏重量并观察其超微结构变化。结果:①与CON大鼠比较,CIHH14大鼠胸腺指数和脾脏指数明显增加;CIHH28和CIHH42大鼠胸腺指数明显增加;急性低氧导致大鼠胸腺和脾脏淋巴细胞受损,CIHH处理大鼠的损伤较CON大鼠明显减轻。②与CON、CIHH28和CIHH42比较,CIHH14大鼠CD8淋巴细胞明显降低,CD4/CD8比值明显升高,NK细胞明显下降;急性低氧导致CON大鼠CD4淋巴细胞升高,CD8淋巴细胞降低,CD4/CD8比值明显升高,NK细胞增加,而对CIHH28和CIHH42大鼠CD4、CD8淋巴细胞和NK细胞无明显影响。③CIHH与急性低氧对大鼠血液IgG无明显影响。④CIHH14大鼠COR较CON、CIHH28和CIHH42大鼠明显增高;急性低氧使CON大鼠EPI、COR、CRP明显增高,而CIHH各组大鼠急性低氧前后EPI、COR、CRP无明显变化。结论:CIHH对机体免疫功能稳态具有调节作用,其作用与神经-内分泌和糖皮质激素有关。Aim：To elucidate the effect of CIHH on cellular immtmity and humoral immunity in rat by using flow cytometry method, immunohistochemistry method and electron microscopy techniques. Methods： Forty-eight male adult Sprague-Dawley rats were randomly divided into 4 groups： control（CON） group, 14 days CIHH （CIHH14） group, 28 days CIHH （CIHH28） group, 42 days CIHH（CIHH42） group. The animals in CIHI-/groups were exposed to 14, 28 and 42 days 113rpobarie hypoxia（simulated 3 000 m altitude, 5 h per day）, respectively. Half of the animals in each group was treated with normaxia and the other half animals were treated with acute hypoxia for 1 h. CD3, CD4, CD8 T lymphocytes, natural killer（NK） cells, IgG, eortisol, epirenamine and C-reactive protein were examined. The weight and ultrastructure of thy- mus and spleen were observed. Results： ①Compared with CON, both indexes of thymus arid spleen in CIHH14 rats were increased significandy. Spleen index, but not thymus index, was increased in CIHH28 and CIHH42 rats. The thymocytes and spleen eytes in rat were injuryed during acute hypoxia, but the damage in CIHH rats was significant slighter than that in CON rats.②Compared with CON, CIHH28 and CIHH42, CD8 in CIHH14 rats were decreased, ratios of CD4/CD8 was increased and NK was decreased.③The rats of CON during acute hy- poxia showed that CD4 was increased, CD8 was decreased, ratio of CD4/CD8 was elevated, and NK was increased. But there were no signifi- cant changes of CD3, CD4, CD8 and NK in CIHH28 and CIHH42 animals during acute hypoxia. ④Compared with CON, CIHH28 and CIHH42, cortisol in CIHH14 rats was increased obviously, Epirenamine, cortisol and C-reactive protein in CON rats were increased, but there were no obvious changes in CIHH rats before and after acute hypoxia. Conclusion： CIHH protects the immune funetion of rat against acute hypoxia, whieh is related with the regulation of neuroendocrine.

关 键 词：间歇性低压低氧 适应 免疫 淋巴细胞 大鼠 

分 类 号：R392.32[医药卫生—免疫学]