消减免疫法制备克伦特罗单克隆抗体  

PREPARATION OF CLENBUTEROL MONOCLONAL ANTIBODY WITH SUBTRACTIVE IMMUNIZATION METHOD

作　　者：李晓丽[1] 李晓芳[2] 宁保安[1] 吴大成 王红勇[1] 陈翔[1] 马新华[1] 欧国荣[1] 高志贤[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津市环境与食品安全风险监控技术重点实验室,天津300050 [2]华中农业大学食品科技学院,湖北武汉430070 [3]广州省农业科学院兽医研究所,广东广州510064

出　　处：《中国应用生理学杂志》2009年第4期573-576,共4页Chinese Journal of Applied Physiology

基　　金：广州市科技攻关重点项目(2006Z2-E0111);天津市重点攻关项目(06YFGZSH02200)

摘　　要：目的:制备克伦特罗(CL)单克隆抗体。方法:重氮化法制备克伦特罗完全抗原,消减免疫法免疫BALB/c小鼠,常规杂交瘤技术制备抗克伦特罗单克隆抗体。结果:消减免疫法使小鼠获得了对BSA的耐受,单抗筛选过程中获得了高达8.2%的阳性率。最后得到了针对CL的特异性抗体。结论:用消减免疫法制备针对小分子污染物的单克隆抗体,可减少在制备单克隆抗体时筛选的工作量,可增加获得预期抗体的机会。Aim： To obtain Clenbuteml monoclonal antibodies. Methyls： Clenbuterol complete antigen was prepared with diazotization method. BALB/c mice was immunized with subtraetive immunization, Clenbuteml monoclonal antibody was prepared with rule hybridoma technique. Results： The mice obtained tolerance to BSA by subtraetive immunization. The rate of the hybridoma cell with positive reaction which had obtained was 8.2%, and the specific elenbuteml monoclonal antibody was obtained at last. Conclusion： Monoclonal antibodies to miemmolecule contaminant be prepared by sub.active immunization, could decrease the workload in the bolting of monoclonal antibodies, and increase the chance to obtain the antibody of expected.

关 键 词：克伦特罗 单克隆抗体 消减免疫 

分 类 号：R392[医药卫生—免疫学]