当归多糖对脐血造血细胞的抗冷冻损伤作用  

Effect of Angelica polysaccharides on resistant ability of cryopreservation damage of UCB hematopoietic cells

作　　者：关雪晶[1] 杨慧[1] 张雁[1] 吴宏[1] 

机构地区：[1]重庆医科大学干细胞与组织工程研究室组织胚胎学教研室,重庆400016

出　　处：《第四军医大学学报》2009年第22期2549-2552,共4页Journal of the Fourth Military Medical University

基　　金：重庆市自然科学基金(CSCT2007BB5284)

摘　　要：目的:探讨当归多糖(APS)对脐血造血细胞的抗冷冻损伤作用.方法:采用细胞计数法比较羟乙基淀粉(HES)、明胶、淋巴细胞分离液(Ficoll)分离脐血单个核细胞(MNC)的回收率.应用细胞计数法、台盼蓝拒染法、集落形成实验和流式细胞术,检测冻存1,3,6mo脐血MNC的生物学活性,将脐血MNC与不同浓度APS共培养24h后冻存,1mo后复苏,观察APS对脐血MNC抗冷冻损伤的作用.结果:HES法、明胶法的MNC回收率均大于85%,显著高于Ficoll法(50.00±4.00)%(P<0.05);冻存1,3,6mo组MNC,CFU-Mix,CD34+细胞回收率及台盼蓝拒染率差异均无显著性,且脐血MNC的损伤与冻存时间不相关.APS50,100,200,400mg/L不同浓度APS组MNC,CFU-Mix回收率和台盼蓝拒染率除APS400mg/L组下降外,其余各组明显上升(P<0.05);各组组间CD34+细胞回收率差异无显著性.结论:APS可有效提高脐血造血细胞的抗冷冻损伤能力.AIM：To explore the effect on umbilical cord blood（UCB）hematopoietic cells of Angelica polysaccharides（APS）to resist cryopreservation damage.METHODS：Cytometry was used to compare the recovery rate of UCB mononuclear cells（MNC）when they were separated respectively by density gradient centrifugation of Ficoll,precipitation method of gelatinum and hydroxyethyl starch（HES）;Cytometry,method of typan blue exclusion,colony-forming assay and flow cytometry were used to detect the bioactivity of UCB MNC after frozen 1,3,6 months;MNC were cultured with various density APS for 24 h,then frozen.one month later thawed and detected the effect of resistant cryopreservation damage of APS on UCB MNC with the same method.RESULTS：Both the recovery rate of method of gelatinum and HES were larger than 85%,and all higher than the one of Ficoll（50.00±4.00）%（P〈0.05）;After 1,3,6 months cryopreservation,there were no significant difference on the recovery rate of UCB MNC,CFU-Mix,CD34＋ cells and rate of typan blue exclusion,the damage was not related with freezing time.In APS groups（except 400 mg/L APS group decreased）,the recovery rate of UCB MNC,CFU-Mix and rate of typan blue exclusion obviously increased（P〈0.05）,but there was no significant difference on the recovery rate of CD34＋ cells.CONCLUSION：APS could enhance the resistant ability of cryopreservation damage of UCB hematopoietic cells.

关 键 词：当归多糖 脐血 造血细胞 冷冻保存 

分 类 号：R285.5[医药卫生—中药学]