双氢青蒿素对前列腺癌裸鼠种植瘤生长及VEGF表达的影响  

Effects of dihydroartemisinin on transplanted prostate cancer in nude mice and expression of VEGF

作　　者：李庆春[1] 高小玲[1] 邹聪[1] 李剑[1] 罗子国[1] 

机构地区：[1]重庆医科大学生命科学研究院,重庆400016

出　　处：《第四军医大学学报》2009年第22期2583-2586,共4页Journal of the Fourth Military Medical University

摘　　要：目的:研究双氢青蒿素(DHA)对前列腺癌PC-3细胞裸鼠种植瘤的抑制作用及对VEGF表达的影响,并探讨其作用机制.方法:采用人前列腺癌PC-3细胞建立裸鼠种植瘤模型,20只模型鼠随机分为对照组;二甲基亚砜(DMSO)溶剂组(DMSO1mL/kg);DHA实验1组(DHA100μmol/kg);DHA实验2组(DHA200μmol/kg).经13d干预后,计算抑瘤率.采用光镜及电镜观察肿瘤组织形态学变化;采用免疫组织化学法检测VEGF的表达水平;采用体视学分析肿瘤微血管密度(MVD).结果:DHA实验1,2组抑瘤率分别为63.186%,69.221%.光镜及电镜下观察可见DHA实验1组和实验2组肿瘤组织内凋亡细胞明显增多,出现典型的凋亡小体.免疫组化结果显示VEGF表达DHA实验1组和实验2组均低于对照组及DMSO溶剂组(P<0.05),但2个DHA实验组组间相比较差异无统计学意义;DMSO溶剂组与对照组组间相比较差异也无统计学意义.2个DHA实验组MVD均低于对照组(P<0.05),但2个DHA实验组之间相比较差异无统计学意义;DMSO溶剂组与对照组组间相比较差异也无统计学意义.结论:DHA具有较强的抗肿瘤作用,其机制与下调凋亡相关基因VEGF有关.AIM：To study the inhibitory effects of dihydroartemisinin（DHA）on the growth of transplantation human prostate cancer PC-3 cells in nude mice and the expression level of VEGF and explore the underlying action mechanism.METHODS：Prostate cancer PC-3 cells were transplanted into 20 nude mice to establishthe solid tumor mode.These nude mice were randomly divided into 4 groups with 5 mice in each group：control group,solvent group（dimethyl sulfoxide 1 mL/kg weight）,1st experimental group（DHA 100 μmol/kg weight）and 2nd experimental group（DHA 200 μmol/kg weight）.The tumor growth inhibition rate was calculated on day 13 after drug administration;Pathomorphism changes of PC-3 cells were observed by light microscope and transmission electron microscope（TEM）after administration of DHA.The positive products of VEGF was tested by immunohistochemical method;Micro-vasvulor density（MVD）was analysed by stereological method.RESULTS：The tumor growth inhibition rates of experimental groups were 63.186% and 69.221%.Apoptotic cells were significantly increased and TEM examination revealedthat the scattered apoptotic bodies were observed in tumor tissues of experimental groups.Immunohistochemical examination revealed that the positive products of VEGF were decreased in experimentalgroups（P〈0.05）;There was no statistically significant difference between 2 experimental groups;There was no statisticallysignificant difference between solvent group and experimental groups,too.MVD was significantly decreased in experimental groups（P〈0.05）;The difference of MVD was not statistical significance between 2 experimental groups;The difference of MVD was not statistical significance between solvent group and experimental groups.CONCLUSION：These results demonstrated that DHA has stronger inhibitory effects on human prostate cancer cell line PC-3 cells in vivo.The action mechanism of DHA might be relatedwith downregulating VEGF.

关 键 词：双氢青蒿素 前列腺癌 凋亡 血管内皮生长因子 微血管密度 

分 类 号：R284.1[医药卫生—中药学] R979.19[医药卫生—中医学]