抗氧化剂对脐血自然杀伤细胞活性及化学发光的影响  

Effect of antioxidants on NK activity and chemiluminescence of umbilical blood

作　　者：赵晋[1] 刘晓菊[2] 郭忠[1] 

机构地区：[1]西北民族大学医学院,甘肃兰州730030 [2]兰州大学第一附属医院,甘肃兰州730000

出　　处：《第四军医大学学报》2009年第22期2654-2656,共3页Journal of the Fourth Military Medical University

摘　　要：目的:探讨褪黑素,亚硒酸钠,N-乙酰半胱氨酸(NAC)及槲皮素对脐血自然杀伤细胞(NK)活性及化学发光的影响.方法:采用四甲基偶氮唑蓝比色法(MTT)进行NK活性测定;化学发光法进行NK细胞杀伤靶细胞过程中发光强度(CL)的测定.结果:褪黑素1～100μmol/L组,亚硒酸钠0.43～6.96μmol/L组可明显增强脐血单个核细胞对K562细胞的杀伤作用;褪黑素10μmol/L组,亚硒酸钠0.87～3.48μmol/L组,槲皮素6.25μmol/L组可明显增强脐血单个核细胞杀伤K562细胞过程中化学CL.褪黑素,亚硒酸钠,NAC及槲皮素等4种抗氧化剂均使化学发光时间延迟,褪黑素组、槲皮素组出现明显的双峰.结论:抗氧化剂对脐血NK细胞具有免疫调控作用,可能与新生儿出生时存在的氧应激状态有关.AIM：To investigate the effect of antioxidants,melatonin,sodium selenite,N-acetyl-cysteine（NAC）and quercetin,on natural killer cell activity（NK activity）and chemiluminescence（CL）of umbilical blood.METHODS：Methlthiazolyl tetrazolium（MTT）method and CL assay were used to detect NK activity and CL intensity during the process of killing target cell.RESULTS：1-100 μmol/L melatonin and 0.43-6.96 μmol/L sodium selenite significantly increased NK activity.CL intensity was greatly enhanced by 10 μmol/L melatonin,0.87-3.48 μmol/L sodium selenite and 6.25 μmol/L quercetin.All the antioxidants delayed the time of CL.Melatonin and quercetin made the CL showed clearly double peaks.CONCLUSION：Antioxidants have the abilityto modulate immune function of NK cell and this maybe relatedto the oxidative state of neonate at birth.

关 键 词：脐血 抗氧化剂 NK活性 化学发光 

分 类 号：Q52[生物学—生物化学]