P68 RNA helicase is a nucleocytoplasmic shuttling protein  

作　　者：Haizhen Wang Xueliang Gao Yun Huang Jenny Yang Zhi-Ren Liu 

机构地区：[1]Departments of Biology, Georgia State University, Atlanta, GA 30303, USA [2]Departments of Chemistry, Georgia State University, Atlanta, GA 30303, USA

出　　处：《Cell Research》2009年第12期1388-1400,共13页细胞研究（英文版）

摘　　要：P68 RNA helicase is a prototypical DEAD box RNA helicase. The protein plays a very important role in early organ development and maturation. Consistent with the function of the protein in transcriptional regulation and pre-mRNA splicing, p68 was found to predominately localize in the cell nucleus. However, recent experiments demon- strate a transient cytoplasmic localization of the protein. We report here that p68 shuttles between the nucleus and the cytoplasm. The nucleocytoplasmic shuttling of p68 is mediated by two nuclear localization signal and two nuclear exporting signal sequence elements. Our experiments reveal that p68 shuttles via a classical RanGTPase-dependent pathway.

关 键 词：P68 RNA helicase nucleocytoplasm shuttle NLS NES DEAD box 

分 类 号：Q959.115.4[生物学—动物学] Q51