Alb4 of Arabidopsis Promotes Assembly and Stabilization of a Non Chlorophyll-Binding Photosynthetic Complex, the CF1CF0-ATP Synthase  

作　　者：Monique Benz Thomas Bals Irene Luise Gugel Markus Piotrowski Andreas Kuhn Danja Schunemann Jurgen Soll Elisabeth Ankele 

机构地区：[1]Department of Biologie I-Botanik, Biozentrum Ludwig-Maximillians-University Munich, Grosshadernerstrasse 2-4, D-82152 Planegg-Martinsried, Germany [2]Munich Centre for Integrated Protein Science CIPSM, Ludwig-Maximillians-University Munich, Feodor-Lynen-Strasse 25, D-81377 Munich, Germany [3]Department of Plant Physiology, Ruhr-University Bochum, D-44780 Bochum, Germany [4]Department of Microbiology, University of Hohenheim, Garbenstrasse 30, D-70599 Hohenheim, Germany

出　　处：《Molecular Plant》2009年第6期1410-1424,共15页分子植物（英文版）

摘　　要：All members of the YidC/Oxal/Alb3 protein family are evolutionarily conserved and appear to function in membrane protein integration and protein complex stabilization. Here, we report on a second thylakoidal isoform of Alb3, named Alb4. Analysis of Arabidopsis knockout mutant lines shows that AIb4 is required in assembly and/or stability of the CF1CF0-ATP synthase （ATPase）. alb4 mutant lines not only have reduced steady-state levels of ATPase subunits, but also their assembly into high-molecular-mass complexes is altered, leading to a reduction of ATP synthesis in the mutants. Moreover, we show that Alb4 but not AIb3 physically interacts with the subunits CF1β and CF0ll. Summarizing, the data indicate that AIb4 functions to stabilize or promote assembly of CF1 during its attachment to the membrane-embedded CF0 part.All members of the YidC/Oxal/Alb3 protein family are evolutionarily conserved and appear to function in membrane protein integration and protein complex stabilization. Here, we report on a second thylakoidal isoform of Alb3, named Alb4. Analysis of Arabidopsis knockout mutant lines shows that AIb4 is required in assembly and/or stability of the CF1CF0-ATP synthase （ATPase）. alb4 mutant lines not only have reduced steady-state levels of ATPase subunits, but also their assembly into high-molecular-mass complexes is altered, leading to a reduction of ATP synthesis in the mutants. Moreover, we show that Alb4 but not AIb3 physically interacts with the subunits CF1β and CF0ll. Summarizing, the data indicate that AIb4 functions to stabilize or promote assembly of CF1 during its attachment to the membrane-embedded CF0 part.

关 键 词：Alb4 Alb3 ARABIDOPSIS thylakoid membrane protein insertion protein complex assembly CF1CF0-ATP synthase. 

分 类 号：Q943[生物学—植物学] Q945.11