喜树碱Pluronic P105/L61混合胶束系统的制备  

Mixed micelles made of Pluronic P105 and L61 as pharmaceutical nanocarriers for camptothecin

作　　者：孙新堂[1] 王金丽[1] 李凌冰[1] 

机构地区：[1]山东大学药学院药剂教研室,济南250012

出　　处：《山东大学学报（医学版）》2009年第11期128-131,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金项目(Y2005C65)

摘　　要：目的制备载有喜树碱的混合胶束系统,增加喜树碱的载药量。方法以Pluronic P105(P105)和Pluronic L61(L61)为材料,难溶性抗癌药物喜树碱为模型药物,制备了载药混合胶束系统。对混合胶束系统的载药量、稳定性进行了研究。采用人胸腺癌细胞系(MCF-7),对载药混合胶束的细胞毒性进行了研究。结果所制备的混合胶束系统,长期存放和多倍稀释后仍然能够稳定存在;加入L61后喜树碱的载药量明显提高。细胞毒性实验结果表明,混合胶束对人胸腺癌细胞系(MCF-7)的细胞毒性高于原料药。结论Pluronic P105/L61混合胶束系统是一种比较稳定的喜树碱给药系统。Objective To increase the drug loading of the poorly soluble anticancer drug,camptothecin（CPT）,micelles made of a mixture of Pluronic P105（P105） and Pluronic L61（L61） were prepared.Methods Mixed micelles made of a mixture of Pluronic P105（P105） and Pluronic L61（L61） were prepared loaded with CPT.The drug loading and colloidal stability of such systems were studied,and cytotoxicity of the CPT-loaded mixed micelles against MCF-7 cancer cells in vitro was determined.Results The CPT-loaded mixed micelles were stable upon storage and dilution.Drug loading of CPT was increased with an increase of the amount of Pluronic L61.Cytotoxicity of the CPT-loaded mixed micelles against MCF-7 cancer cells in vitro was remarkably higher than that of the free drug.Conclusion Mixed micelles made of a mixture of Pluronic P105（P105） and Pluronic L61（L61） may serve as a stable delivery system for poorly soluble CPT.

关 键 词：胶束 喜树碱 细胞毒性 

分 类 号：R944.9[医药卫生—药剂学]