多聚甲醛固定法检测MGC803^T细胞膜表面分子功能的研究  

Paraformaldehyde-Fixed Assay for Testing CytotoxicActivity Releted to Membrane of MGC T803Cells

作　　者：胡荫[1,2] 尹维刚[1,2] 许佐良[1,2] 

机构地区：[1]包头医学院基础部 [2]北京医科大学

出　　处：《包头医学院学报》1998年第2期1-2,共2页Journal of Baotou Medical College

摘　　要：用插入突变的膜结合型ＴＮＦ－α基因的重组逆转录病毒载体转染人胃癌细胞ＭＧＣ８０３，获得膜结合型ＴＮＦ－α基因转染的阳性人胃癌细胞ＭＧＣ８０３（ＭＧＣＴ８０３）。用多聚甲醛固定ＭＧＣＴ８０３及未转染的人胃癌组胞ＭＧＣ８０３（ＭＧＣＮ８０３），检测其细胞膜表面ＴＮＦ－α的细胞毒活性。结果表明，ＭＧＣＴ８０３细胞对鼠纤维肉瘤细胞Ｌ９２９有杀伤作用，而对照细胞ＭＧＣＮ８０３无杀伤作用，说明逆转录病毒介导的膜结合型ＴＮＦ－α基因在人胃癌细胞中获得有效表达，多聚甲醛固定后ＭＧＣＴ８０３细胞膜表面ＴＮＦ－α活性仍然存在。认为多聚甲醛固定法用于检测细胞膜表面分子功能，方法简便、易行、敏感。 We used retroviral vector LXSN to construct recombinant retroviral vector with mutant membrane bound TNF-α gene.The retroviral vectors were used to transduced the human gastric cancer cell line,MGC803cells and positive colonies were obtained(named as MCG T803).MCGT803cells and control were fixed with paraformaldehyde prior to their incubation with target cells.Paraformaldedhyde-fixde MCG T803cells remained cytotoxic in a dose-dependent manner to L929 cells. The results suggest that mutant TNF-α gene could be efficently expression in the transduced human gastric cancer cells and paraformaldehyde-fixed MCG T803cells remained membrane cytotoxin related to TNF-α.

关 键 词：多聚甲醛 细胞膜表面分子 MGC803 细胞因子 

分 类 号：R446.1[医药卫生—诊断学] R730.2[医药卫生—临床医学]