我国Ⅱ型糖尿病患者载脂蛋白A-Ⅳ多态性的研究  

A study on the polymorphism of apolipoprotein A-Ⅳ in Chinese type 2 diabetic patients

作　　者：郭改娥[1] 刘德文[2] 沃兴德[3] 

机构地区：[1]山西长治医学院生物化学教研室,046000 [2]山西医科大学生物化学教研室 [3]浙江中医学院分子医学研究所

出　　处：《中国糖尿病杂志》1998年第3期150-153,共4页Chinese Journal of Diabetes

摘　　要：用等电聚焦电泳（IEF）和Western－blot技术检测125例Ⅱ型糖尿病患者血清apoA－Ⅳ的基因频率、表型分布和血清水平，以了解Ⅱ型糖尿病患者apoA－Ⅳ的多态性及其与apoA－Ⅳ血清水平的关系。结果显示：Ⅱ型糖尿病患者apoA-Ⅳ的两个常见等位基因频率为：apoA-Ⅳ*1=0.9960，apoA-Ⅳ*2=0；两个常见表型的分布为：apoA－Ⅳ1-1=99．2％，apoA－Ⅳ2-1=0；apoA－Ⅳ的血清水平为0.0262±0．0125g／L，与健康人群组相比较，apoA－Ⅳ多态性没有显著性差异（P＞0．75，P＞0．25），其血清水平显著增高（P＜0．001）。结论；Ⅱ型糖尿病患者apoA－Ⅳ的多态性不是发病的决定因素。To study apoA-Ⅳ polymorphism and its relation to serum isoelectric focusing (IEF) and immuno-apoA-Ⅳ level in type 2 diabetes patients,serum apoA-Ⅳ level,gene frequency and phenotype distribution were determined by blotting technique in patients from Hangzhou. The results showed that the frequency of apoA-Ⅳ. 1 and apoA-Ⅳ. 2,two common alleles was 0. 996 and 0 respectively. The distribution of apoA-Ⅳ 1-1 and apoA-Ⅳ 2-1, the two common phenotypes was 99. 2 % and 0 respectively. The serum level of the apoA-Ⅳ in type 2 diabetes was 26. 2 ±12. 5mg/dl. No statistically significant difference was seen in apoA-Ⅳ allele frequencies and phenotype distribution between normal Chinese and type 2 diabetic patients. Serums apoA-Ⅳ concentration was significantly higher in diabetes than in normal individuals(P <0.001). It is concluded that the apoA-Ⅳ polymorphism is similar in the two populations and that the apoA-Ⅳ polymorphism has no significant impact on serum apoA-Ⅳ level.

关 键 词：载脂蛋白A-IV 多态性 基因频率 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]