反相高效液相色谱法测定艾司唑仑血药浓度  

Determination of Estazolam in Plasma by RP-HPLC with UV Detection

作　　者：杨春霞[1] 何粤红[1] 于海燕[1] 郭亚东[2] 

机构地区：[1]云南省精神病医院,云南昆明650224 [2]昆明医学院药学院,云南昆明650031

出　　处：《中国民族民间医药》2009年第23期32-33,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：建立艾司唑仑血药浓度的测定方法。使用反相高效液相色谱法,采用Herpesil ODS C18柱(250mm×4.0mm,5(m);流动相为甲醇-乙腈-水(40∶20∶40);流速为:1.0mL/m in;检测波长240nm。艾司唑仑的线性范围为50-1000ng/mL,r=0.9977,平均加样回收率96.41%。本法操作简单、准确,可以应用于临床监测艾司唑仑血药浓度。To develop a HPLC method for the quantitative determination of estazolam in plama. The residues were analyzed with a RP- HPLC system. Herpesil ODS C18 column （250mm ×4. 0mm, 5 （m） with the mobile phase consisted of methanol- acetonitrile- water （40： 20： 40）. The flow rate was 1. 0mL/min and the detection wavelength was at 240nm. The calibration curve was proved to be linear in the range of 50 -1000ng/mL, r =0. 9977. The average recovery of estazolam was 96. 41% . The method provides a simple and accurate way for clinical monitoring of estazolam in plasma.

关 键 词：反相高效液相色谱法 艾司唑仑 血药浓度 

分 类 号：R927.2[医药卫生—药学]