阿司匹林-烟酰胺-锌络合物对胃癌细胞SGC-7901的生长抑制作用  

Inhibitory effect of aspirin-niacinamide-zinc complex on human gastric carcinoma cell line SGC-7901

作　　者：蒲其松[1] 郑倩[2] 张建武[1] 

机构地区：[1]川北医学院药理教研室 [2]川北医学院机能实验中心

出　　处：《中国临床药理学与治疗学》2009年第10期1091-1094,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：四川省教育厅重点项目(2005A080)

摘　　要：目的:探讨阿司匹林-烟酰胺-锌络合物(商品名:佛立沙,WUY)对人胃癌细胞SGC-7901增殖的抑制作用。方法:用MTT法考察WUY对人胃癌细胞SGC-7901增殖的抑制作用,用流式细胞技术检测WUY对胃癌细胞周期的影响。结果:WUY在2、4、8、16mmol/L剂量时对胃癌SGC-7901细胞的增殖抑制率分别为9.95%、19.28%、37.36%、63.47%(P<0.05),平均IC50为11.06mmol/L。增殖抑制曲线表明,WUY对胃癌细胞有抑制作用且呈明显的量效和时效关系。流式细胞仪检测显示,WUY对胃癌细胞作用24h后,呈浓度依赖性改变细胞周期的分布,G0/G1期细胞比例下降,S期细胞比例和G2/M期细胞比例升高。结论:WUY能显著抑制人胃癌细胞SGC-7901的增殖,其作用可能与影响细胞周期有关。AIM：To investigate the inhibitory effect of aspirin-niacinamide-zinc complex （Wuyisa,WUY） on human gastric carcinoma cell line SGC-7901.METHODS：The inhibitory effect of WUY on SGC-7901 cells was evaluated by MTT analysis.The effect of WUY on SGC-7901 cell cycle was determined with flow cytometry.RESULTS：After treated with 2,4,8,16 mmol/L of WUY,the inhibition rates were 9.95%,19.28%,37.36%,63.47% （P〈0.05）.The average IC50 of SGC-7901 cells was 11.06 mmol/L.The dose effect relationship and time effect relationship were described in the growth curve.The cell cycle distribution was changed in a concentration-dependent manner,after gastric cancer cell was treated for 24 hours with WUY by flow cytometry.Among phases of cell cycle,the G0/G1 phase ratio was decreased and the S and G0/M phase ratio were increased.CONCLUSION：WUY can obviously inhibit the growth of human gastric carcinoma cell line SGC-7901 cells.It may be associated with the phases of cell cycle.

关 键 词：阿司匹林.烟酰胺.锌络合物 SGC-7901细胞 抑制作用 细胞周期 

分 类 号：R971.1[医药卫生—药品] R965.1[医药卫生—药学]