应用Dot-ELISA进行日本血吸虫循环抗原检测时其滴度与感染度关系的动态观察  

DYNAMIC OBSERVATION ON THE RELATIONSHIP BETWEENSCHISTOSOMA JAPONICUM INFECTIOSITY AND ITS TITERSOF CIRCULATING ANTIGENS DETECTED WITH DOTELISA*

作　　者：刘世国[1,2,3] 宋力[1,2,3] 谢来平 韩家俊[1,2,3] 邓伟文 石佑恩[1,2,3] 

机构地区：[1]新乡医学院寄生虫学教研室 [2]新乡医学院微免教研室 [3]同济医科大学寄生虫学教研室

出　　处：《中国寄生虫病防治杂志》1998年第3期204-206,共3页Chinese Journal of Parasitic Disease Control

基　　金：联合国开发计划署;世界银行及WHO热带病研究和培训特别规划的部分资助

摘　　要：以２０条／只和１００条／只剂量的尾蚴感染家兔，并于第９ｗｋ用吡喹酮对约半数家兔进行一次性灌胃治疗，依此建立日本血吸虫感染模型。用分别定位于日本血吸虫表皮膜、肠道上皮和虫卵的单克隆抗体建立直接斑点酶联免疫吸附试验（Ｄｏｔ－ＥＬＩＳＡ）并应用该法分别检测感染家兔血中３类循环抗原、膜相关抗原（ＭＡＡ），肠相关抗原（ＧＡＡ）和可溶性虫卵抗原（ＳＥＡ），观察循环抗原滴度水平和日本血吸虫感染度之间的关系。结果发现，当用Ｄｏｔ－ＥＬＩＳＡ法检测３类循环抗原时，２０条尾蚴感染组与１００条尾蚴感染组家兔不论治疗与否，血中３类循环坑原滴度水平无显著性差异。Halves of two groups of rabbits infected separately with two doses of Schistosoma japonicum(each rabbit infected with 20 or 100 cercariae) were treated with praziquentel(220 mg/kg body weight given as a single dose via stomach)at the 9th week after infection. In order to analyse the relationship between titers of circulating antigens(CAg) and infectiosity, we developed the DotELISA based on the application of 3 McAbs to detect respectively the gutassociated antigen(GAA), membrane associated antigen(MAA) and solube egg antigen(SEA) of Schistosoma japonicum. The results showed that the titers of CAgs in sera between the two groups of rabbits had no significant difference, whether they were treated or not. Therefore,DotELISA to detect CAgs is not suitable for judgement of infectiosity of Schistosoma japonicum.

关 键 词：日本血吸虫 循环抗原 DOT-ELISA 感染度 

分 类 号：R383.24[医药卫生—医学寄生虫学]