纳米级磁性多柔比星脂质体的制备  

Preparation and property of magnetic doxorubicin nanoliposomes

作　　者：肖勇[1] 陶凯雄[1] 王虎[1] 夏泽锋[1] 王国斌[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,武汉430022

出　　处：《山东医药》2009年第46期18-19,共2页Shandong Medical Journal

基　　金：国家"863"重点基金资助项目(2001AA218051)

摘　　要：目的制备纳米级磁性多柔比星脂质体(DOX-MLP)。方法用反相蒸发法制备纳米级DOX-MLP,以正交实验设计确定最佳制备工艺。电镜观察脂质体形态,zetapal粒度分析仪测量其粒径,紫外sephadex G-50法测定其包封率,磁测仪测量其磁响应性。结果DOX-MLP呈圆形或椭圆形,粒径为(293.4±20.6)nm,包封率为30.8%±3.27%,磁响应性(50.28±7.8)emu。结论用反相蒸发法制备的磁性多柔比星脂质体平均粒径小,磁响应性高,达到纳米级标准。Objective To prepare magnetic doxorubicin （DOX） nanoliposomes. Methods Magnetic DOX liposomes （DOX-MLP） were prepared by the reverse-phase evaporation method,an optimal technique was chosen with orthogonal design, and its diameter distribution was observed under electronic microscope. Its encapsulation efficiency was detected by the sephadex column-ultraviolet method ,the magnetic properties of the DOX-MLP were measured with a magnetometer. Result The DOX-MLP were globular or elliptic, the average particle size was （293.4 ＋ 20.6） nm, its encapsulation efficiency was 30.8% ＋ 3.27%, and its average magnetic response was （50.28 ＋ 7.8） emu. Conclusion The DOX-MLP prepared by thereverse-phase evaporation method have litter diameter and high magnetic response.

关 键 词：磁性 纳米脂质体 反相蒸发法 包封率 磁响应性 

分 类 号：R541.4[医药卫生—心血管疾病]