鲤鱼发育早期HPG轴和GH/IGF轴相关因子的转录起始分析  

GENE EXPRESSION PROFILES OF GROWTH AND REPRODUCTION RELATED GENES DURING THE EARLY DEVELOPMENT OF COMMON CARP(CYPRINUS CARPIO L.)

作　　者：卢月娇[1,2] 胡炜[1] 朱作言[1] 

机构地区：[1]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,武汉430072 [2]中国科学院研究生院,北京100049

出　　处：《水生生物学报》2009年第6期1126-1131,共6页Acta Hydrobiologica Sinica

基　　金：国家973计划(2007CB109206);国家自然科学基金(30771641)资助

摘　　要：采用RT-PCR的方法,以不同发育时期的鲤鱼胚胎和幼鱼为材料,研究了与鱼类生殖相关的HPG轴以及与生长相关的GH/IGF轴中GnRH、GtH以及GH、GHR和IGF重要信号分子的转录起始特征。结果显示,sGnRH、cGnRH、GtH-Iβ亚基和GHR于鲤鱼胚胎受精后20h开始转录,IGF-1于受精后23h开始转录,GtH-IIβ亚基于受精后26h开始转录,GtHα亚基于受精后46h开始转录,GH于1dph(孵出后第1天)开始转录。其中,GHR和IGF-1均早于GH开始转录,GtHα亚基和β亚基的转录起始时间不同步。研究结果为揭示鲤鱼生殖与生长间的调控机制积累了重要的科学依据。As many researches revealed some relations between HPG and GH/IGF axis in teleost,we detected gene expression profiles of growth-and reproduction-related genes from HPG or GH/IGF axis in common carp（Cyprinus carpio） embryos and early larval stages,in order to find some regulatory relations between HPG and GH/IGF axis in the early development stages of common carp.The genes under detection were sGnRH,cGnRH,GtH α subunit and β subunit from HPG axis and GH,IGF,growth hormone receptor（GHR） from GH/IGF axis,with β-actin gene as the internal control.Samples were collected from embryos and larvae of unfertilized eggs,64-cell stage,32-cell stage,256-cell stage,50%-epiboly,20-,23-,26-,32-,40-,46-and 58-hours post fertilization（hpf）,and 1-,2-,3-,5-,7-and 9-days post hatching（dph） reared at 23℃,and then RNA extracted from the samples was used in RT-PCR.sGnRH,cGnRH,GtH α subunit,GtH-I β subunit,GtH-II β subunit,GH,GHR and IGF-1 showed different expression patterns in carp embryos and larvae.Development around 20h post fertilization（hpf） and 23hpf was critical for carp embryos,as sGnRH,cGnRH,GtH-I β subunit and GHR were first detectable at 20hpf,IGF-1 at 23hpf and GtH-II β at 26hpf.Meanwhile,embryos in 20hpf and 23hpf stages showed apparent development in brain region,indicating a regulatory function of GnRH and GtH in the development of brain.GtH α subunit was first detectable at 46hpf while GH was first detectable at 1d post hatching（dph）.The difference in the timing of the expression of GH and IGF mRNA may suggest that IGF gene expression is not GH-dependent.Additionally,the timing of the expression of GtH α subunit,GtH-I β subunit,GtH-II β subunit were unsynchronized in embryos,suggesting a regulatory function of GtH α subunit in GtH expression.

关 键 词：GnRH/GtH GH/IGF/GHR 鲤鱼胚胎 转录起始 

分 类 号：Q344.1[生物学—遗传学]