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作 者:张磊[1] 樊启学[1] 方巍[1] 赵志刚[2] 李波[1] 杨凯[1] 董俊锋[1]
机构地区:[1]华中农业大学水产学院,武汉430070 [2]大连水产学院生命科学与技术学院,大连116023
出 处:《水生生物学报》2009年第6期1152-1159,共8页Acta Hydrobiologica Sinica
基 金:湖北省科技攻关计划项目(2006AA201B05)资助
摘 要:在孵化环道连续微流水培养、水温(24±2)℃条件下,斑鳜(Siniperca scherzeri Steindachner)初孵仔鱼全长为(4.87±0.10)mm(n=50),卵黄囊体积为(1.461±0.172)mm3(n=50),油球直径为(0.47±0.04)mm(n=50)。仔鱼孵出12h,胸鳍增大,具有一定阵发性水平游动能力,1日龄巡游模式建立;2日龄口膜消失,开始主动摄食,进入混合营养期,3日龄外源性摄食关系完全建立。5日龄仔鱼的卵黄和油球全部消失,进入外源营养期;15日龄全长达到(13.72±0.76)mm(n=12)。仔鱼发育过程中,其全长生长存在内源性营养阶段的较快速生长,混合营养阶段的慢速生长以及外源性营养阶段的快速生长三个生长期相,平均增长率为0.59 mm/d,对仔鱼全长TL(mm)与日龄D(d)进行回归,其生长模型为:TL=-0.0004D3+0.0283D2+0.2159D+4.9335(R2=0.985,n=261)。2—15日龄,口宽与全长呈正比关系。仔鱼从初孵到PNR仅为5—6d,具有摄食能力的时间4d,仔鱼依赖外源性营养开始时间较早,对饥饿的耐受力较差。Feeding and Growth of Siniperca scherzeri larvae were studied in function morphology and ecology,and the growth model of larvae was established,which could provide biological and ecological data for Siniperca scherzeri larvae rearing.Experiments were performed in the Hubei Qichun Chidonghu Seed Stock Farm from 3rd May to 6th June,2007.Adult fish,Siniperca scherzeri,were caught in the Zhelin Lake of Jiangxi Province.44 adult fish Siniperca scherzeri(♀: 34,♂: 10)whose gonads reached full development were chosen to obtain larvae by artificial propagation at 17:00 on 4th May.The eggs were collected in a circular-racy-way to hatch with slow flow.The hatchery circular-racy-way was maintained at temperatures of(24±2)℃,DO(6.51±1.32) mg/L,COD(4.88±0.94) mg/L,pH 7.54±0.31,TN(0.63±0.27) mg/L and TP(0.08±0.01) mg/L.82 percentage collected eggs were hatched at 6:00 on 11th May.The newly-hatched larvae were cultivated in a circular-racy-way for feeding tests.From 17:00 to 20:00 on 12th May,16000 newly-hatched larvae were collected,placed and cultivated in the other circular-racy-way for starvation tests to measure point-of-no-return(PNR).At least 10 larvae were picked from feeding tests in 8:00-10:00 everyday to observe their growth by anatomy microscope(NIKON SMZ2800),and the total length(TL) and volume of the yolk sac of larvae were measured in the process of observation.When the larvae started exogenous feeding and established exogenous nutrition,the growth was observed every 5-6h.At last,the daily rate of growth,the volume of the yolk sac and the consuming rate of yolk sac of larvae were figured out,and the growth model of larvae was established.The initially feeding rates of larvae were calculated in the feeding tests.The point of no return(PNR) was defined as a critical time at which the initially preying larvae could tolerate starvation.The PNR was measured as follows: the initially feeding rate of larvae was daily measured and the maximum feeding rat
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