光学相差显微镜下前庭囊斑两型毛细胞的形态学鉴别  

Comparative morphology of the two type's hair cells from saccule and utricle under inverted phase contrast microscope

作　　者：康喜讯[1] 孔维佳[2] 张松[2] 郭长凯[2] 王彦君[2] 朱云[2] 夏交[2] 

机构地区：[1]东莞市人民医院耳鼻咽喉科,广东东莞523000 [2]华中科技大学同济医学院附属协和医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉头颈外科杂志》2009年第23期1082-1085,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家自然科学基金资助项目(No:39670781)

摘　　要：目的:探讨电生理实验中,光学相差显微镜下较为可靠的前庭两型毛细胞辨别标准。方法:对于豚鼠椭圆囊和球囊分离所得的前庭毛细胞,分别对细胞胞体的长、宽以及表皮板纤毛的长度进行测量,并对长宽比和纤毛胞长比进行分析。结果:豚鼠椭圆囊和球囊的两型前庭毛细胞相比,细胞的宽度和纤毛的长度比较差异无统计学意义,Ⅰ型毛细胞的长度明显较长,长宽比较大,纤毛胞长比较小。结论:在既往以是否有明显的颈部来区分两型前庭毛细胞的基础上,结合细胞的长宽比和纤毛胞长比进一步进行辨别,有助于更准确地区分两型毛细胞。Objective：To explore more reliable standards for identifying vestibular hair cells of saeeule and utricle prepared in studies with patch clamp technique under inverted phase contrast microscope. Method：The length and width of two type＇s hair cell＇s were measured besides the length of cilia, and all datas were analyzed statistically. Result：The width and length of cilia of two type＇s hair cells in saccule and utricle from guinea pig were similar. The length of type I was longer than that of type Ⅱ, so the ratio between length and width was larger and the ratio of the length between cilia and cell body was small. Conclusion： Two type＇s hair cells of saccule and utriele from guinea pig may be distinguished through the ratio of celt body＇s length and width even the ratio of the length be-tween cilia and cell body, besides the standards before.

关 键 词：毛细胞 形态 椭圆囊 球囊 

分 类 号：R764[医药卫生—耳鼻咽喉科]