IL-8／CXCR2轴促进兔内皮祖细胞迁移、增殖及VEGF的表达  

IL-8/CXCR_2 axis promotes proliferation,migration and VEGF expression of rabbit endothelial progenitor cells in vitro

作　　者：毛梅[1,2] 吕学军[1] 王艺[1] 徐剑铖[1] 

机构地区：[1]第三军医大学新桥医院全军呼吸内科研究所,全军呼吸病研究重点实验室,重庆400038 [2]广州军区武汉总医院呼吸内科,武汉430070

出　　处：《第三军医大学学报》2009年第24期2429-2431,共3页Journal of Third Military Medical University

摘　　要：目的观察不同浓度白介素-8(interleukin-8,IL-8)对体外培养兔外周血内皮祖细胞(endothelial progenitor cells,EPCs)生物学功能的影响,探讨趋化因子IL-8/CXCR2轴对EPCs血管生成功能的作用。方法密度梯度离心法获取兔外周血单个核细胞(mononuclear cells,MNCs),接种至纤维连接蛋白(fibronectin,FN)包被的培养板上,培养7～10d后收集贴壁细胞鉴定EPCs,并用不同浓度IL-8的培养基继续培养24h后采用MTT比色法、改良的Boyden小室实验,观察EPCs的迁移和增殖能力;采用RT-PCR、Western blot方法检测CXCR2、VEGF mRNA及蛋白表达水平。结果IL-8显著增加兔外周血EPCs的增殖和迁移能力(P<0.05),且随着IL-8浓度升高而增强。IL-8促进CXCR2膜受体和VEGF的表达,且随着IL-8浓度增加而增加。结论IL-8/CXCR2轴能显著促进EPCs的迁移、增殖及VEGF的表达。Objective To investigate the proliferation, migration and VEGF expression of rabbit peripheral blood endothelial progenitor cells （EPCs） after the treatment of interleukin-8 （IL-8） at different concentra- tions. Methods Total mononuclear cells （MNCs）were isolated from rabbit peripheral blood by density gradi- ent centrifugation, and then the cells were plated on fibronectin-coated culture dishes. After cultivation for 7 d, the attached cells were incubated with IL-8 in a series of concentrations for 24 h. The proliferation and migration activities of EPCs were measured with MTT assay and modified Boyden chamber assay, and reverse transcription （RT）-polymerase chain reaction （PCR） and Western blot analysis were used to detect the mRNA and protein expressions of CXCR2 and VEGF of EPCs. Results The proliferation, migration and CXCR2 and VEGF expressions of EPCs were regulated by IL-8, and enhanced with the increasing concentration of IL-8. Conclusion The IL-8/CXCR2 axis can significantly promote the EPCs proliferation, migration, and VGEF expression.

关 键 词：发夹型DNA探针 SM耐药rpsL43密码子 荧光分光光度仪 液相杂交 

分 类 号：R322.12[医药卫生—人体解剖和组织胚胎学] R329.28[医药卫生—基础医学]