恶性肿瘤患者不同病期阶段外周血单核细胞CD14和TLR4的表达研究  

The Study on the Expression of CD14 and TLR4 in Peripheral Blood Monocytes during the Different Stage of Cancer Patients

作　　者：蔡智慧[1] 裴毅[2] 

机构地区：[1]山西医科大学,太原030013 [2]山西省肿瘤医院老年科,太原030013

出　　处：《医学研究杂志》2009年第12期36-38,共3页Journal of Medical Research

基　　金：中华人民共和国民政部;中国老年学学会"十一五"专项研究课题[民人教科字(2007)18-2-14]

摘　　要：目的探讨恶性肿瘤患者不同病期外周血单核细胞 CD14和 TLR4(Toll 样受体4)的表达。方法采用流式细胞术检测50例健康体检者(对照组)及80例恶性肿瘤患者(按照影像学观察到肿瘤大小变化和相关肿瘤标志物的测定值分为稳定期及进展期)外周血单核细胞 CD14和 TLR4的表达。结果恶性肿瘤患者外周血单核细胞 CD14稳定期及进展期的表达水平分别为(6.1600±2.32217)%、(5.8629±1.91896)%,与健康体检者(对照组)(5.5580±1.42272)%比较,无统计学意义,恶性肿瘤患者稳定期 TLR4表达水平为(10.4444±3.16225)%与健康体检者(对照组)的(8.8780±5.24612)%比较无统计学意义;进展期 TLR4表达水平为(14.7286±6.95213)%,明显高于健康体检者(对照组)(8.8780±5.24612)%(P<0.05)和恶性肿瘤患者稳定期(10.4444±3.16225)%(P<0.05)。结论单核细胞表面的 TLR4的表达水平在恶性肿瘤患者进展期较稳定期及对照组显著增高,而 CD14的表达与病期无关,与对照组无差异。因此在 CD14处于稳定状态下,TLR4的表达上调有可能预测肿瘤患者病情恶化。Objective To explore the expression of CD14 and TLR4 （Toll like receptor - 4） in peripheral blood monocytes during the different stage of cancer patients. Methods The expression of TLR4 and CD14 on peripheral blood monocytes was determined by flow cytometry in 50 healthy control group and 80 patients with malignant tumor who were divided into stable group and advanced group according to the change of the size of tumor measured by image and/or the outcome of tumor makers. Results No significant difference was found in the expression level of CD14 between the different stage of malignant tumor （stable group and advanced group）and healthy controls [（6.1600%±2.32217% ,5.8629%±1.91896% ） vs （5.5580%±1.42272% ）]. The expression of TLR4 in stable group had no significant difference as compared with control group[（10.4444± 3.16225）% vs （8.8780±5.24612）%]. The expression of TLR4 in advanced group was significantly higher than that in controls and stable group [（14.7286±6.95213 ） % vs （8.8780±5.24612） % , （10.4444±3.16225）%）（P〈0.05）]. Conclusion In the advanced group,the expression of TLR - 4 in peripheral blood monoeytes was significantly higher than the others. The expression of CD14 had no relationslvip betaveen the three groups. Therefore , when CD14 was in the stable state, increased expression level of TLR4 could predict the patients＇ condition getting worse.

关 键 词：恶性肿瘤 单核细胞 CDL4 TLR4 

分 类 号：R512.62[医药卫生—内科学] R-05[医药卫生—临床医学]