HPLC法测定胸腺五肽乙酯在血浆中的稳定性  

Study on the Stability of Thymopentin Ethyl Ester in Rat Plasma by HPLC

作　　者：迟强[1] 栾中山[1] 邵全胜[1] 杨志[1] 

机构地区：[1]中国人民解放军第406医院215临床部,大连116041

出　　处：《医学研究杂志》2009年第12期66-68,共3页Journal of Medical Research

摘　　要：目的研究胸腺五肽乙酯在血浆中的稳定性。方法由于胸腺五肽乙酯在血浆中半衰期较短,为保证药物检测顺利进行,血浆以生理盐水进行稀释,以1,10-菲罗啉作为酶抑制剂,并对孵育后的加药血浆进行冰浴处理,采用HPLC法测定胸腺五肽及胸腺五肽乙酯在血浆中的半衰期。色谱条件为:C_(18)柱(150×4.6mm),流动相:胸腺五肽乙酯:甲醇一磷酸盐缓冲液30:70(pH3.0,内含0.2%三乙胺);胸腺五肽:甲醇一磷酸盐缓冲液10:90(pH7.0)。检测波长:220nm、275nm。结果在稀释后的血浆中,胸腺五肽的半衰期为1.3min,而胸腺五肽乙酯的半衰期为2.0min。结论酯化物在血浆中的半衰期略有延长。Objective To study the stability of thymopentin ethyl ester（TP - ET） in rat plasma. Methods Thymopentin ethyl ester degraded quickly in plasma. In order to perform the assay successfully, saline was used to dilute the rat plasma. 1,10 -phenanthroline was adopted as enzyme inhibitor and the plasma containing TP - ET was cooled down after incubation for scheduled time. The half-lives of thymopentin and its ethylester were determined by HPLC. The chromatograph condition as follows： C lscolumn（ 150×4.6mm） , mobile phase ： methanol - phosphate buffer solution 30：70 （ pH3.0, containing 0.2% triethylamine） for TP - ET, methanol - phosphate buffer solution 10：90 （ pH 7.0） for thymopentin; detection wavelength 220nm,275nm. Results The half - lives of thymopentin and its ethylester were 1.3 min and 2.0 min. Conclusion The half- life of thymopentin ester in the rat plasma was a little longer.

关 键 词：胸腺五肽乙酯 HPLC 稳定性 

分 类 号：R512.62[医药卫生—内科学] R286.0[医药卫生—临床医学]