仙草提取物对CCl_4致小鼠肝损伤的保护作用  

Protective Effects of Extracts of Mesona blumes on Carbon Tetrachloride Induced Liver Injury in Mice

作　　者：刘小玲[1] 李艳[1] 赵鹏[2] 覃辉艳[2] 

机构地区：[1]广西大学轻工与食品工程学院,广西南宁530004 [2]广西壮族自治区疾病预防控制中心卫生毒理与功能检验科,广西南宁530021

出　　处：《食品与生物技术学报》2009年第6期768-772,共5页Journal of Food Science and Biotechnology

基　　金：广西壮族自治区自然科学基金项目(0728009)

摘　　要：将70只雄性昆明小鼠随机分成7组:正常对照组、模型对照组、阳性对照组、醇提组、水提低、中、高剂量组。醇提组按500 mg/kg灌胃仙草醇提物,低、中、高剂量组分别按200、500、1000mg/kg灌胃仙草水提物,阳性对照组按200 mg/kg灌胃联苯双酯,正常对照组和模型对照组以等体积蒸馏水灌胃,连续30 d。以CCl4制成小鼠肝脏损伤模型,检测并比较各组小鼠血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性和血清白蛋白(Alb)、甘油三酯(TG)含量,肝组织匀浆中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)活性。仙草水提物低、中、高剂量组与模型对照组相比均能显著降低ALT(P<0.05)、AST、TG值(P<0.01),显著增加Alb含量(P<0.05),极显著降低小鼠肝组织匀浆MDA值(P<0.01),极显著升高GSH、GSH-PX值(P<0.01),对SOD酶活性(P>0.05)无显著影响;同时,仙草水提物低、中、高剂量组与联苯双酯阳性对照组相比各指标无显著差异(P>0.05),而仙草醇提物与模型对照组相比上述各生化指标无显著差异(P>0.05)。仙草水提物对CCl4所致的小鼠肝损伤具有一定保护作用。The objective of this study was to explore the protective effects of Mesona blumes extracts on carbon tetrachloride induced liver injury in mice. Seventy male mice were randomly divided into seven groups （model control group, positive control group, ethanol extracts group, low dosage, middle dosage and high dosage of water extracts groups）. The mice in the above groups were fed for 30 days. ALT, AST, Alb, TG in serum and SOD, MDA, GSH, GSH-PX in liver tissue were measured and compared. It was found that water extracts of Mesona blumes significantly increased the values of AST （P〈0. 01）, 〈0.01） in serum and MDA （P〈0.01）, GSH, GSH- ALT （P〈0. 05）, Alb（P〈0.05）, TG （P PX （P〈0.01） in liver tissue, respectively. However, no obvious difference was detected at the SOD activities （P〉0.05）. Also, There is no remarkable difference between different dosage of water extracts groups and bifendate pills groups（P〉0.05）. Ethanol extracts of Mesona blumes wasn＇t found to effectively decreased liver damage in terms of above mentioned biochemical parameters compare to model control group. Those results indicated that water extracts of Mesona blumes plays a significant protective role on liver injury which induced by carbon tetrachloride.

关 键 词：仙草 提取物 肝损伤 CCL4 保护作用 

分 类 号：R285.5[医药卫生—中药学]