雷公藤优良无性系组织培养技术的研究  

Study on tissue culture conditions and technology of Tripterygium wilfordii superior clone

作　　者：李建鹃[1,2] 洪伟[1,2] 吴承祯[1,2] 谢安强[1,2] 李键[1,2] 林晗[1,2] 

机构地区：[1]福建农林大学森林生态研究所,福建福州350002 [2]福建省高校森林生态系统过程与经营重点实验室,福建福州350002

出　　处：《福建林学院学报》2009年第4期315-319,共5页Journal of Fujian College of Forestry

基　　金：国家教育部博士点基金资助项目(20061038901);福建省科技重大专项资助项目(2006NZ0001)

摘　　要：以雷公藤绿色嫩叶诱导产生的愈伤组织为外植体,通过正交试验设计,研究了不同培养基对雷公藤优良无性系不定芽诱导、不定芽增殖和生根的影响。结果表明:不同浓度的激素组合对雷公藤愈伤组织不定芽诱导培养的影响显著,最优诱导培养基为MS+IAA 0.2 m.gL-1+KT 0.5 m.gL-1+6-BA 1.5 m.gL-1;不同浓度的激素组合对雷公藤不定芽增殖培养的影响极显著,最佳增殖培养基为MS+NAA 0.1 m.gL-1+KT 0.1 m.gL-1+6-BA 1.0 m.gL-1;不同培养基对雷公藤生根培养的影响不显著,生根培养适合的培养基为1/2MS+NAA 2.0 m.gL-1+KT 0.1 m.gL-1。By orthogonal experiment the tender green leaves were used as explants to study the effect of different medium on adventitious buds induction, adventitious buds proliferation and rooting experiment of Tripterygium wilfordii superior clone. The results showed that there was a significant impact to T. wilfordii adventitious buds induction cultivated on different concentrations of hormone combinations. The optimal induction medium was MS ＋IAA 0.2 mg·L^-1 ＋ KT 0.5 mg·L^-1 ＋6-BA 1.5mg·L^-1. There was a very significant impact to T. wilfordii adventitious buds proliferation cultivated on different concentrations of hormone combinations. The optimal proliferation medium was MS ＋ NAA 0.1 mg·L^-1 ＋ KT 0. 1 mg·L^-1 ＋ 6-BA 1.0 mg·L^-1. There was not significant impact to T. wilfordii rooting cultivated in different treatments, the adaptive rooting medium was 1/2MS ＋ NAA 2.0 mg·L^-1＋ KT0.1 mg·L^-1.

关 键 词：雷公藤 不定芽诱导 不定芽增殖 生根培养 

分 类 号：S722.37[农业科学—林木遗传育种]