SOD-PVP/Au电极的电化学行为及对H_2O_2的电催化  

Electrochemical behaviors of SOD-PVP/Au electrode and its electrocatalysis determination for H_2O_2

作　　者：何晓英[1] 华俊[1] 杨洪川[1] 谭宝玉[1] 蒋河[1] 

机构地区：[1]西华师范大学化学化工学院,四川南充637002

出　　处：《化学研究与应用》2009年第12期1623-1626,共4页Chemical Research and Application

基　　金：四川省教育厅重点研究课题项目(2004A106)

摘　　要：以聚乙烯吡咯烷酮K30为电催化剂，羧甲基纤维素为膜固定剂，将超氧化物歧化酶固定在电极上，制备了SOD-PVP／Au修饰电极。采用循环伏安法研究了该修饰电极的电化学行为，在pH7．0 PBS缓冲溶液中于0．305V和0．111V处出现一对明显的氧化还原峰，电极反应是一个受扩散控制的准可逆过程，扩散系数4．71×10^-7cm^2／s、异相电子迁移常数5．37×10^-6cm／s。修饰电极能够催化H2O2的电还原，还原峰电流与H2O2浓度在2．0×10^-6～2．0×10^-4mol／L范围呈线性关系，相关系数R=-0．99042，可用于H2O2的电催化检测。A SOD-PVP/Au electrode was prepared by mixing superoxide dismutase （SOD）with ployvinylpyrrolidone K30 （PVP）on gold electrode. The electrochemical behaviors of SOD-PVP/Au were studied by cyclic voltammetry in the presence of 0.1 mol/L PBS buffer solution（ pH =7.0）. The results indicated that the modified electrode can realize the direct electrochemistry of SOD and the electrode process was reversible one and controlled by difusion. A good linear relation was found between the peak currents of SOD- PVP/Au and the concentration of H2O2 in range of 2.0×10^-6～2.0×10^-4mol/L, with correlation coefficient of -0. 99042. This method was applied to the analysis and determination of H2O2.

关 键 词：超氧化物歧化酶 电化学行为 过氧化氢 

分 类 号：O657.1[理学—分析化学]