围生期双酚A暴露对雄性子代脑组织神经营养因子-3表达的影响  

Effects of perinatal exposure to bisphenol A on expression of NT-3 of weaning male offspring in rats

作　　者：龙冬梅[1] 吴德生[2] 

机构地区：[1]成都军区疾病预防控制中心,四川成都610021 [2]四川大学华西公共卫生学院,四川成都610041

出　　处：《西南国防医药》2009年第12期1165-1167,共3页Medical Journal of National Defending Forces in Southwest China

基　　金：国家自然科学基金重点课题(30030120)

摘　　要：目的:研究围生期母鼠双酚A(BPA)暴露对断乳期雄性子代大鼠脑组织神经营养因子-3(NT-3)表达的影响,探索BPA影响脑发育的机制。方法:将妊娠母鼠从妊娠第11 d直至产后断乳期进行BPA灌胃染毒,剂量浓度分别为100、20、2 mg/(kg.d);对照组则用同体积的食用色拉油灌胃。在子代大鼠出生后第21 d,将雄性仔鼠断头处死,迅速取脑组织进行免疫组织化学染色,检测NT-3蛋白表达。结果:免疫组化结果显示,NT-3在海马锥体细胞和大脑皮质神经元都有表达,BPA暴露的各剂量组子代NT-3表达减弱,与对照组比较有显著差异(P<0.05)。结论:围生期母鼠暴露于BPA可引起雄性子代脑中NT-3表达下调,这可能是BPA影响脑发育的机制之一。Objective ： To study the effects of perinatal exposure to bisphenol A （BPA） on the expression of neurotrophin - 3 （ NT - 3 ） in the brain of F1 male offspring and explore the influence mechanism of BPA on brain development. Methods： Pregnant SD rats were given BPA at 2,20,100 mg/kg body weight per day,respectively,since the eleventh day of gestation throughout the whole lactation by gastric garage until their pups were weaned on postnatal dav 21. FI male pups in each group were killed on postnatal day 21 and their brains were moved to detect the expression of NT - 3 protein by immunohistochemical staining. Results： lmmunohistochemical staining demonstrated that NT - 3 protein expression was found in both hippocampal pyramidal cells and cerebral cortical neurons and its expression in all BPA - treatment dosage groups decreased significantly compared with the control group（P 〈 0.05）. Conclusion：Perinatal exposure to BPA may cause a down - regulation of NT - 3 expression in the brain of the male offspring,which may be one of mechanisms of effects of BPA on brain development.

关 键 词：环境内分泌干扰物 双酚A 神经营养因子-3 脑发育 

分 类 号：R994.6[医药卫生—毒理学]