枳壳质量标准研究  

Quality standard of Fructus Aurantii

作　　者：王发英[1] 徐欢[1,2] 杨武亮[1] 杨世林[1] 魏玲[1] 陈海芳[1] 袁金斌[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004 [2]江西中医学院附属医院,江西南昌330006

出　　处：《中成药》2009年第12期1897-1901,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(30660230);国家药典委员会2010版一部标准研究:枳壳药材(饮片)质量标准研究(YD-186;YD-187;YD-188)

摘　　要：目的:完善枳壳的质量标准。方法:以柚皮苷和新橙皮苷为对照品,对枳壳建立TLC鉴别,采用高效液相色谱法测定枳壳中柚皮苷和新橙皮苷的含量。柚皮苷和新橙皮苷的含量测定采用Hypersil C18色谱柱(200mm×4.6mm,5μm),流动相为乙腈-水(磷酸调pH=3)(20:80),检测波长为283nm,流速:1.0mL/min;柱温:25℃。结果:薄层色谱鉴别特征斑点明显。含量测定中柚皮苷进样量在0.2～2.0μg范围内呈良好的线性关系,r=0.9999,平均加样回收率为100.12%,RSD为0.74%;新橙皮苷进样量在0.2～2.0μg范围内呈良好的线性关系,r=0.9998,平均加样回收率为99.82%,RSD为1.42%。结论:本法专属性强,重复性好,可用于枳壳的质量控制。AIM：To perfect the quality standard of Fructus Aurantii. METHODS：The reference substances consisted of naringin and neohesperidin,TLC was used to identify naringin and neohesperidin from Fructus Aurantii,and the contents of naringin and neohesperidin were determined by HPLC.The Hypersil C 18 （200 mm×4.6 mm,5 μm）was used,mobile phase was acetonitrile-0.5% H3PO4（20：80）,detection wavelength was at 283 nm,flow rate was 1.0 mL/min. RESULTS：The TLC spots developed were fairly and simply identical.The concentration of naringin showed a good linear relationship at the range of 0.2-2.0 Ixg,r =0.999 9 ,the average recovery was 100.12% ,RSD = 0.74%. The concentration of neohesperidin showed a good linear relationship at the range of 0.2- 2.0 μg,r =0. 999 8 ,the average recovery was 99.82% ,RSD = 1.42%. CONCLUSION： The methods are special and accurate, and can be used for the quality control of Fructus Aurantii.

关 键 词：枳壳 质量标准 柚皮苷 新橙皮苷 TLC HPLC 

分 类 号：R284.1[医药卫生—中药学]