镉对硒引起的离体大鼠肝细胞DNA损伤作用的影响  

Effect of Cadmium on Selenium-induced DNA Damage in Rat Liver Cells

作　　者：余日安[1] 陈学敏[1] 吴志刚[1] 杨成峰[1] 

机构地区：[1]同济医科大学环境卫生学教研室

出　　处：《中国公共卫生学报》1998年第5期292-294,共3页

基　　金：国家自然科学基金

摘　　要：应用单细胞凝胶电泳研究氯化镉对亚硒酸钠引起的大鼠肝细胞ＤＮＡ损伤作用的影响。结果表明，在８７５μｍｏｌ／Ｌ、１７５０μｍｏｌ／Ｌ、３５００μｍｏｌ／Ｌ的剂量下，亚硒酸钠单独作用时，可引起肝细胞ＤＮＡ损伤。当氯化镉与亚硒酸钠联合作用时，８７５μｍｏｌ／Ｌ的氯化镉对８７５μｍｏｌ／Ｌ、１７５０μｍｏｌ／Ｌ、３５００μｍｏｌ／Ｌ的亚硒酸钠引起的ＤＮＡ损伤存在明显的拮抗作用，而３５００μｍｏｌ／Ｌ的氯化镉对８７５μｍｏｌ／Ｌ、１７５０μｍｏｌ／Ｌ、３５００μｍｏｌ／Ｌ亚硒酸钠引起的ＤＮＡ损伤不但未显示出拮抗作用，反而使得ＤＮＡ损伤程度加重。１７５０μｍｏｌ／Ｌ的氯化镉与１７５０μｍｏｌ／Ｌ的亚硒酸钠联合作用，拮抗作用最为明显。本研究结果提示，在一定剂量条件下，氯化镉对亚硒酸钠引起的大鼠肝细胞ＤＮＡ损伤存在拮抗作用。Using single cell gel electrophoresis or Comet assay,this article presents the effect of cadmium on selenium-induced DNA damage in rat liver cells.The results show that at the concentration of 8 75μmol/L,17 50μmol/L,35 00μmol/L,selenium can induce DNA damage and has obvious doseeffect relationship.When cadmium acts with selenium jointly,cadmium,at the concentration of 8 75μmol/L,can antagonize seleniuminduced DNA damage significantly.But at the concentratoin of 35 00μmol/L,cadmium don't antagonize selenium-induced DNA damage,but intensify the damage.When both cadmium and selenium are at the concentration of 17 50μmol/L,cadmium has the most significant antagonism effect on seleniuminduced DNA damage.This research infers that at certain dose,cadmium can antagonize seleniuminduced DNA damage in rat liver cells.

关 键 词：镉 硒 DNA损伤 拮抗作用 肝细胞损伤 

分 类 号：R995[医药卫生—毒理学] R992[医药卫生—药学]