不同治法方药对心肌缺血再灌注损伤大鼠防治作用的对比研究  

Contrast Observation on Protective and Therapeutic Effects of Different Therapies and Formulas on Myocardial Ischemia Reperfusion Injury in Rats

作　　者：唐丹丽[1] 张华敏[2] 孙明杰[1] 隋宇[1] 

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]中国中医科学院中医药信息研究所

出　　处：《国际中医中药杂志》2009年第6期485-486,共2页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目（项目编号：30701066）

摘　　要：目的观察中医不同治法方药对心肌缺血再灌注损伤（MI／RI）大鼠的防治作用。方法将60只雄性SD大鼠随机分为5组：假手术组、模型组、祛痰宽胸组、活血化瘀组及痰瘀同治组。可逆性冠脉左前降支结扎缺血30min再灌注2h复制MI／RI模型，检测再灌注后各组大鼠血清乳酸脱氢酶（LDH）和肌酸激酶（CK）的活性，电镜下观察心肌形态学改变以及用免疫组化法测定各组心肌TNF—α、ICAM-1蛋白表达的变化。结果与模型组比较，各治疗组均能降低血清LDH、CK水平并抑制TNF—α、ICAM-1蛋白表达，减轻心肌超微结构的损伤，且以痰瘀同治组疗效最为显著。结论祛痰宽胸法、活血化瘀法及痰瘀同治法均对MI／RI大鼠有保护作用，且以痰瘀同治法疗效最明显。Objective To investigate the protective effects of different Chinese medical treatments on myocardial ischemia-reperfusion injury in rats. Methods 60 SD rats were divided into 5 groups randomly： a sham-operated group, a model group, a removing phlegm and enlarging chest group, an activating blood and dissolving stasis group, and a treating both phlegm and blood stasis group. The model of MI/RI of the myocardium was reproduced by ligation of left descending artery for 30rain followed by releasing the ligation for 2 hours in rats. Serum contents of LDH-L, CK were measured, TNF-α and ICAM-1 expressions in myocardium were determined with immunohistochemistry and myocardial ultrastructure at the ischemia region was observed with the transmission electron microscope after myocardial reperfusion injury. Results Compared with the model group, LDH-L, CK and TNF-α, ICAM-I levels were lower, myocardial ultrastructural changes were improved in all the other four groups treated by different Chinese medicine（P〈0.01 or P〈0.05）, especially in the group treating both phlegm and stasis. Conclusion The removing phlegm and enlarging chest method, activating blood and dissolving stasis method, treating both phlegm and blood stasis method can protect myocardium from MI/RI, especially the method of treating both phlegm and blood stasis.

关 键 词：心肌缺血再灌注损伤 痰瘀同治法 

分 类 号：R28[医药卫生—中药学]