成骨细胞在表面酰胺化聚乳酸膜上的黏附和增殖行为  

Adhesion and Proliferation of Osteoblast on the Amidation Surface of Polylactide Film

作　　者：田冶[1,2] 王迎军[1,2] 周长忍[3] 曾庆慧[3] 谭帼馨[4] 

机构地区：[1]华南理工大学教育部特种功能材料实验室 [2]材料科学与工程学院,广州510640 [3]暨南大学材料科学与工程系,广州510630 [4]广东工业大学轻工化工学院,广州510006

出　　处：《高等学校化学学报》2009年第12期2491-2495,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:50732003,50830101,50803018);广东省自然科学基金(批准号:4205786)资助

摘　　要：利用紫外光接枝聚合丙烯酰胺(Acrylic amide,AAm)获得表面酰胺化的聚乳酸(PLA)膜,并考察了成骨细胞在酰胺化表面的黏附和增殖行为.结果表明,酰胺基的引入改善了PLA膜的表面亲水性,其表面水接触角由78°减少到56°,自由能由42.7mJ/m2增大到51.4mJ/m2;与对照组相比,成骨细胞在改性表面培养3d后有大量的丝状伪足伸出,并且较快地进入了细胞分裂期,表明PLA膜表面的酰胺化能够促进细胞的黏附和增殖.Acrylic amide（AAm） was polymerized onto polylactide（PLA） film via UV irradiation to achieve amidation surface, furthermore the adhesion and proliferation of osteoblast on this surface was investigated. The results show that the hydrophilicity of PLA film is improved by introduction amido： the contact angle decreases from 78° to 56° and the free energy increases from 42.7 mJ/m2 to 51.4 mJ/m2. Compared with the control, osteoblast spread out more filopodia after 3 d incubation on amidation surface, and it entered cell division phase more quickly, which indicated that the surface amidation could promote the cell′s adhesion and proliferation on the PLA film.

关 键 词：聚乳酸 酰胺化表面 接枝聚合 黏附和增殖 

分 类 号：O631[理学—高分子化学] O647.11[理学—化学]