凉血化瘀方对人视网膜色素上皮细胞吞噬作用影响的研究  

Study on the Effect of Liangxue Huayu Decoction on Phagocy tosis in Cultured Human Retinal Pigment Epithelium Cells

作　　者：于静[1] 唐由之[2] 冯俊[1] 陈燕云[3] 张励[1] 

机构地区：[1]中国中医科学院眼科医院,北京100040 [2]中国中医科学院,北京100700 [3]北京同仁医院,北京100730

出　　处：《辽宁中医杂志》2009年第12期2038-2041,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30472229)

摘　　要：观察凉血化瘀方对体外培养的人视网膜色素上皮(retinal pigment epithelial,RPE)细胞吞噬作用的影响。方法:将人RPE细胞随机分为5组,分别为空白对照组、凉血化瘀方2mg/mL、200μg/mL、20μg/mL及2μg/mL组。密度梯度离心法提取光感受器外节(rod outer segment,ROS),并用FITC标记。将标记成功的ROS加入各组细胞,在相同的条件下共同培养12h。终止培养后,在荧光显微镜下每张片子随机选取10个视野进行照相,用Laica Q Win图像分析软件进行分析,并用面积加以标准化(单位:μm2)。结果:实验结果示凉血化瘀方2mg/mL组细胞吞噬ROS荧光面积(34.890±9.148)μm2、凉血化瘀方200μg/mL组细胞吞噬ROS荧光面积(34.272±4.801)μm2,明显多于空白对照组(26.643±5.076)μm2。余各组与空白对照组比较未见明显差异。结论:合适浓度的凉血化瘀方中药具有提高体外培养的人RPE细胞吞噬作用的功能。Objective：To investigate the effect of the LiangXue HuaYu decoction to the phagocytosis of cultured human retinal pig, ment epithelial（RPE） cells in vitro. Methods：The rod outer segments （ROS） were labeled by FITC. Human RPE cells were divided into 5 groups, To feed them with different concentration of liang xue hua yu decoction （2mg/mL,200μg/mL,20μg/mL and 2μg/mL）. All the grouPs were incubated with same quantity of FITC labled ROS. and then were rinsed at the same incubation time to terminate the p.hagoeytosis. Samples were photoed by a fluorescent microscope. Images were analyzed with Lecia Q - Win image analysis software. The quantity of the ingested ROS was analyzed by the quantification of FITC - fluorescence emission. Results ：The phagocytosis of the RPE cells feeded with 2mg/mL and 200μg/mL Liang Xue Hua Yu decoction were more than the group feed with no Liang Xue Hua Yu decoction. Conclusion ： LiangXue HuaYu decoction of suitable concentration could enhance the phagoeytosis of RPE cells.

关 键 词：视网膜色素上皮细胞 吞噬 中医药 凉血化瘀方 

分 类 号：R285.5[医药卫生—中药学]