补肾中药对肾阳虚小鼠模型影响的脑基因图谱研究  

Gene Expression Profile Study on the Cerebral Gene in Model Mouse with Kidney-yang Deficiency Treated by Kidney Tonifying Drugs

作　　者：杨裕华[1,2] 李震[1] 李文[2] 

机构地区：[1]山东中医药大学,山东济南250014 [2]山东省疾病预防控制中心,山东济南250014

出　　处：《辽宁中医杂志》2009年第12期2174-2178,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30672573)

摘　　要：目的:该研究旨在基因水平上探讨补肾中药金匮肾气丸、右归丸对"劳倦过度、房事不节"肾阳虚小鼠模型"以药反证"影响的药物作用机理。方法:对照组、造模组、金匮肾气丸组和右归丸组,各组雄性小鼠分别为10只、15只、10只和10只。4组均予正常喂养,对照组和造模组每天灌胃蒸馏水0.5mL/只,两治疗组每天分别灌胃金匮肾气丸混悬液0.5mL/只和右归丸混悬液0.5mL/只,造模组和两治疗组均采用雄雌鼠比例1∶6同笼喂养和雄鼠每日游泳30～40min达4周,以诱导产生"劳倦过度、房事不节"肾阳虚证。用36K mouse genome array鼠脑芯片检测正常对照组和肾阳虚模型组鼠脑基因,并以二者荧光信号相对强度比值≥2和≤0.5筛选差异显著基因,并进一步用qRT-PCR对部分差异表达基因进行验证。结果:造模组诱导成功"劳倦过度、房事不节"肾阳虚鼠模型,造模组/对照组上调基因而两治疗组/造模组基因下调的有23个基因,其主要是与炎症/免疫、神经传递/信号转导等相关基因;造模组/对照组下调基因而治疗组/造模组基因上调的基因有6个,其主要是与细胞周期/细胞结构、神经传递/信号转导、转录等有关基因。qRT-PCR也证实了金匮肾气丸组/造模组、右归丸组/造模组和造模组/对照组的GH和Scgb3a1等6个差异基因表达。结论:金匮肾气丸、右归丸可使肾阳虚小鼠模型显著下调的激素、黑色素浓集素显著上调,并促进细胞增殖,从而在基因水平上探讨了金匮肾气丸和右归丸的药物作用机理。Objective：To inquire into the cerebral gene change of the model mouse with kidney-yang deficiency caused by excessive physical and sexual activities and its improved syndrome change due to using drugs of efficacy such as Jinkuishenqiwan and Youguiwan which reinforcing the kidney-yang according to traditional Chinese medicine, the mechanism of the medicine. Methods：There were four groups randomly：the control group which has 10 male mice,the model group which has 15 male mice, the Jinkuishenqiwan treatment group which has 10 male mice and the Youguiwan group which has 10 male mice. All of them were fed normally, and perfused with 0. 5mL of distill water for each mouse in the control group and model group, and with 0. 5mL suspension of the drugs for each in the treatment groups every day. The experimental mice in the model group and the treatment groups were kept with six female mice each in the same cage, and all of the male mice swam until they submerged and scooped up from water everyday,which lasted four weeks. The mice might induce the kidney-yang deficiency because of excessive physical and sexual activities. The brain gene of the mice between control group, model group and treatment groups was detected with a mouse brain gene chip of 36K Mouse genome array,which screened the differentially expressed genes according to the ratio equal to or above 2 and equal to or below 0. 5 with the related fluorescent intensity comparing with the two groups＇ ,part of which could be further verified by means of qRT-PCR. Results ：The animal model of kidney-yang deficiency in the mode/group was induced by way of excessive physical and sexual activities successfully, there were twenty three genes among the upregulated genes in the model group versus control group but down-regulated in the treatment group versus model group,mainly involving the related genes of inflammation/immunization and neurotransmissions/signal transduetion. There were six genes among the down-regulated genes in the model group versus control group

关 键 词：DNA芯片 肾阳虚 动物模型 金匮肾气丸 右归丸 

分 类 号：R332[医药卫生—人体生理学]