外磁场作用下特异性磁性NICD对脊髓神经干细胞增殖与凋亡的影响  

Effects of specific magnetic notch intracellular domain under the outer magnetic field on proliferation and apoptosis of spinal cord neural stem cells

作　　者：张衣北[1] 夏春[1] 陈安民[2] 

机构地区：[1]厦门大学附属中山医院骨科,厦门361004 [2]华中科技大学同济医学院附属同济医院骨科,武汉430030

出　　处：《华中医学杂志》2009年第6期297-299,共3页Central China Medical Journal

基　　金：福建省卫生厅青年基金资助项目(2007-1-48)

摘　　要：目的研究Notch信号通路在脊髓损伤部位高表达后,对内源性脊髓神经干细胞再生能力的影响,探讨其在脊髓损伤修复中的作用。方法构建鼠NICD(Notch intracellular domain,NICD)质粒,用经典的氧化还原法将NICD质粒结合至葡聚糖磁性纳米颗粒(dextran magnetic nanoparticles,DMN)上,参照Alivisatos和Conwell方法构建纳米化目的基因,在外磁场作用下转染体外培养鼠脊髓神经干细胞,检测转染前后细胞增殖与凋亡情况。结果纳米化NICD-DMN转染体外培养内源性脊髓神经干细胞后,细胞明显增多,而凋亡明显减少,统计学有显著意义(P<0.05)。结论特异性磁性NICD在外磁场协助下明显促进内源性脊髓干细胞增殖,抑制其凋亡。Objective To investigate the effects of the high expression of Notch signaling pathway in injured spine on the regeneration of the endogenous neural stem cells, and explore its role in the repair of spinal cord injury. Methods The mouse Notch intracellular domain （NICD） plasmids were constructed, and integrated to dextran magnetic nanoparticles （DMN） according to the classical oxidation-reduction method. By using the Alivisatos, and Conwell methods, Nano-based target gene was built, and transfected into rat spinal cord neural stem cells cultured in vitro under the outer magnetic field. Proliferation and apoptosis of cells were detected before and after transfection. Results After the transfection of NICD-DMN into the spinal stem cells, the number of cells was increased significantly, while the apoptosis decreased （P〈0.05）. Conclusion In combination with the outer magnetic field, the specific magnetic NICD can significantly promote the proliferation of endogenous spinal cord stem cells, and inhibit their apoptosis.

关 键 词：脊髓神经干细胞 纳米颗粒 NICD 

分 类 号：R651.2[医药卫生—外科学]