双灭活原生质体融合构建纤维燃料乙醇全糖发酵高产菌株研究  

Construction of Strain Capable of Co-fermenting Pentose and Hexose by Diinactivated Protoplast Fusion for Producing Fuel Ethanol from Cellulose

作　　者：宋安东[1] 谢慧[1] 王风芹[1] 张建[1] 

机构地区：[1]河南农业大学生命科学学院,河南郑州450002

出　　处：《林产化学与工业》2009年第6期20-24,共5页Chemistry and Industry of Forest Products

基　　金：国家农业成果转化资金项目(2006GB2D000173);河南省教育厅2006度高校青年骨干教师资助(无编号)

摘　　要：以嗜鞣管囊酵母P-01(Pachusolen tannophilus)和南阳酒精酵母1308(Saccharomyces cerevi-siae)的单倍体D-12为亲本,通过双灭活原生质体融合技术,获得一株能够进行全糖发酵的稳定的酵母融合株F10,发酵醪液乙醇体积分数达到1.3%,比亲株P-01的0.95%提高了36.8%。对玉米秸秆水解液进行发酵表明,融合株F10发酵玉米秸秆时乙醇体积分数最高可达1.1%,远高于亲株。A stable fusant F10 which can co-ferment pentose and hexose into fuel ethanol was obtained by diinactivated protoplast fusion between Pachusolen tannophilus P-01 and haploid D-12 of Saccharomyces cerevisiae 1308. The ethanol concentration of FIO in fermentation broth reached 1.3 %, or 36.8 % higher than parent strain P-01 （0.95 % ）. The fermentation activity of F10 in corn-stalk hydrolyzates was analyzed, the ethanol concentration of FIO reached 1.1% （volume fraction）, which was far higher than the parent strains.

关 键 词：双灭活 原生质体融合 全糖发酵 燃料乙醇 

分 类 号：TQ353[化学工程]