毛细管电泳-电化学发光间接测定人血浆中盐酸甲氯芬酯  

Indirect Determination of Meclofenoxate Hydrochloride in Human Plasma by Capillary Electrophoresis Coupled with Electrochemiluminescence Detection

作　　者：陆桦[1] 徐泉秀[1] 施爱红[2] 李林秋[2] 邓必阳[1,2] 

机构地区：[1]桂林工学院材料与化学工程系,广西桂林541004 [2]广西师范大学化学化工学院,广西桂林541004

出　　处：《分析科学学报》2009年第6期639-642,共4页Journal of Analytical Science

基　　金：广西壮族自治区研究生教育创新计划(No.2007106020703M61)

摘　　要：建立了一种毛细管电泳-电化学发光间接测定人血浆中盐酸甲氯芬酯（MFX）的新方法。在碱性条件下，三联吡啶钌在铂电极上的弱电化学发光信号可以被MFX增敏，且MFX水解后灵敏度比未水解的灵敏度提高了6倍。研究了工作电极电位、磷酸盐缓冲溶液浓度及其pH值、分离电压、进样电压和进样时间等实验参数对MFX水解产物N，N-二甲氨基乙醇测定的影响。在优化实验条件下，其浓度线性范围为0.020～50μg／mL，检出限（3σ）为6．3ng／mL，峰高的相对标准偏差为3．1％（10μg／mL MFX，n=11）。利用该法间接地测定了人血浆中MFX的含量，回收率为95．9％～96．9％．An indirect determination of meclofenoxate hydrochloride （MFX） in human plasma was developed by using capillary eleetrophoresis coupled with eleetrochemiluminescence （ECL） detection. The ECL intensity was obviously increased by hydrolysis product of MFX. The effects of different NaOH concentrations on hydrolysis ratios were examined and the operating parameters that affected the separation and detection were optimized. Under the optimal conditions, the linear range for MFX was from 0.01 to 50 μg/mL （r^2=0. 9996） with a detection limit of 6. 3 ng/mL （3σ）. The method was successfully applied for the determination of meclofenoxate hydrochloride in human plasma with the recoveries varying from 95.9 to 96.9%.

关 键 词：毛细管电泳 电化学发光 盐酸甲氯芬酯 人血浆 

分 类 号：O657.8[理学—分析化学]