原花青素对H_2O_2致PC12细胞氧化损伤作用的保护研究  

Protective effect of procyanidine on PC12 cells injury induced by H_2O_2

作　　者：蒋国军[1] 路明珠[2] 伊佳[2] 

机构地区：[1]浙江萧山医院药剂科,浙江杭州311201 [2]第二军医大学长征医院药学部,上海200433

出　　处：《药学实践杂志》2009年第6期414-416,420,共4页Journal of Pharmaceutical Practice

基　　金：杭州市科技发展计划社会发展科研攻关项目(20070733B09)

摘　　要：目的:探讨原花青素对H2O2致PC12细胞氧化损伤的保护作用。方法:使用H2O2造成PC12细胞损伤,MTT法测细胞活性,流式细胞仪检测细胞凋亡率,免疫组化法检测细胞中Bax与Bcl-2的蛋白表达。结果:原花青素能提高H2O2损伤后细胞的活性;能减少细胞凋亡;能够增加Bcl-2蛋白表达,减少Bax蛋白表达。结论:原花青素对H2O2致细胞损伤具有保护作用,能够减少细胞凋亡,其机制可能与其增加Bcl-2蛋白表达,减少Bax蛋白表达有关。Objective：To investigate the protective effect of procyanidine on the injury of PC12 cells induced by H2O2. Methods： The PC12 cells were injuried induced by H2O2. The cell activity was determined by MTT. The cell apoptosis rate were detected by flow cytometry. Proteins of Bcl-2 and Bax were detected by immunohistochemistry. Results： Procyanidine increased the survival rate and decreased the apoptotic rate of PC12 induced by H2O2. Procyanidine increased Bcl-2 expression and decreased of Bax expression in PC12 induced by H2O2. Couclusion：Procyanidine can inhibit the apoptosis of PC12 induced by H2O2, which might be correlated with increasing Bcl-2 expression and decreasing Bax expression.

关 键 词：原花青素 PC12细胞 细胞凋亡 

分 类 号：R931.71[医药卫生—生药学] R965[医药卫生—药学]