分泌型磷脂酶A_2在人皮肤黑素瘤细胞和组织中的表达和分布  

Expression and distribution of secretory phospholipase A_2 in human skin melanoma cells and tissues

作　　者：张立超[1] 张永佳[1] 顾伟鹰[1] 王慧菁[1] 时扣荣[1] 

机构地区：[1]上海市第七人民医院药剂科,上海200137

出　　处：《药学实践杂志》2009年第6期417-420,共4页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金项目(30472103);浦东新区卫生系统学科带头人项目(PWRd2008-07);浦东新区科委科研项目(PKJ2009Y23)

摘　　要：目的:比较分泌型磷脂酶A2(sPLA2)在人恶性黑素瘤A375细胞、黑素瘤组织与健康人皮肤细胞、组织中的表达和分布。方法:分别采用ELISA法和Western Blot法检测细胞上清液和细胞内sPLA2的表达,免疫组化法考察人皮肤组织中sPLA2的表达和分布。结果:恶性黑素瘤A375细胞上清液的sPLA2浓度为(177.27±13.57)pg/mL,明显高于人表皮HaCaT细胞的(21.42±5.05)pg/mL(P<0.05)和真皮成纤维细胞的(2.75±1.23)pg/mL(P<0.05);其细胞内sPLA2/GAPDH的灰度比值为7.03±1.31,亦明显高于HaCaT细胞的1.45±0.37(P<0.05)和成纤维细胞的0.31±0.11(P<0.05);黑素瘤组织中呈现大量sPLA2阳性细胞和颗粒,而健康人表皮和真皮组织中则未见该阳性细胞和颗粒。结论:sPLA2在人恶性黑素瘤A375细胞和黑素瘤组织中较健康皮肤细胞和组织均有较高表达和分布,提示sPLA2有可能成为皮肤黑素瘤靶向给药的新靶点。Objective ： To investigate the expression and distribution of secretory phospholipase A2 （ sPLA2 ） in A375 human malignant melanoma cells and melanoma tissues compared with normal human skin cells and tissues. Methods ： The expression of sPLA2 in the clear supernatant and intra-cellular of cells was detected by ELISA method and Western Blot method, respectively. Immunohistochemical method was used to investigate the expression and distribution of sPLA2 in the human skin tissues. Results： The levels of sPLA2 in the clear supernatant of A375 malignant melanoma cells was （ 177.27 ± 13.57 ） pg/mL, which was significantly higher （P 〈 0.05 ） than the group of human epidermal HaCaT cells （21.42 ± 5.05 ） pg/ml and the group of dermal fibroblast cells （2.75 ± 1.23 ） pg/mL, respectively. The ratio of sPLA2/GAPDH in the A375 cells was （7.03 ± 1.31 ）, which was also significantly higher （P 〈 0. 05 ） than the group of human epidermal HaCaT cells （1.45 ± 0.37） and the group of dermal fibroblast cells （0.31 ± 0.11 ）, respectively. Many sPLA2 positive cells and granules were presented in the tumor tissues. In contrast, positive cells and granules above were unobserved in the normal human epidermal and dermal tissues. Conclusion：sPLA2 is expressed and distributed at higher levels in A375 human malignant melanoma cells and melanoma tissues compared with the normal skin cells and tissues, indicating the sPLA2 maybe a novel trigger for targeted drug delivery to skin melanoma.

关 键 词：分泌型磷脂酶A2 黑素瘤 表达 分布 

分 类 号：R965.1[医药卫生—药理学]