牙鲆胚胎冷冻损伤的观察  

Observation of injury flounder(Paralichthys olivaceus)embryos during cryopreservation

作　　者：王春花[1] 田永胜[2] 

机构地区：[1]淮南师范学院生命科学系,安徽淮南232001 [2]青岛黄海水产研究所重点开放实验室,山东青岛266071

出　　处：《生物学杂志》2009年第6期5-7,共3页Journal of Biology

基　　金：国家自然科学基金项目(30570259);安徽省高校青年教师资助计划项目(2008jq1134)

摘　　要：为了观察牙鲆胚胎在冷冻保存过程中的形态受损情况,将其浸入20%PM(20%丙二醇和20%甲醇1∶1的混合液)中平衡,并用程序化法和玻璃化法对其冷冻保存2h后解冻,用摄影显微镜记录其在抗冻剂里平衡时和冷冻保存后的形态。结果显示在平衡过程中胚胎卵膜出现凹陷(称为溶液损伤),但可以恢复;在冷冻过程出现胞内冰损伤,是致命的;用程序化法冷冻保存的尾芽期胚胎卵膜和卵黄完好,胚体边缘受伤;用玻璃化法保存的尾芽期胚胎,卵膜和卵黄损伤较重,胚体损伤较轻;因此将玻璃化法和程序化法结合可以达到扬长避短的效果。In order to observe the change of injury flounder embryos during cryopreservation, flounder embryos were immerged in 20% PM （ the mixed solution of 20% propylene glycol and 20% methanol with ratio of 1 ： 1 ） firstly, and cryopreserved in LN2 （ liquid nitrogen） for two hours with programmed method and vitrification respectively, then thawed, and taken photos with micro-camera. Normally embryos were injured for the osmosis （ named solution damage ） and the intracellar ice （ named intraeellar ice damage ） , when iramerged in 20% PM, this injury can be cured. When the embryos were cryopreserved by a programmed method, the ehorion and yolk of embryos were in good condition, and the cell part was damaged. Correspondingly, when cryopreserved by vitrification, the chorion and yolk was damaged, and the cell part was better. So combining the two methods would remedy the cryopreservation.

关 键 词：牙鲆 胚胎 溶液损伤 胞内冰损伤 

分 类 号：Q954.4[生物学—动物学]