高效液相色谱法测定复方银杏通脉胶囊中丹参酮ⅡA的含量  

Determination of the content of tanshenone ⅡA in Co Yinxing Tongmai capsule by HPLC

作　　者：罗茂玉[1] 陈登丰 

机构地区：[1]福建龙岩市第一医院,龙岩364000 [2]福建南平市人民医院,南平353000

出　　处：《海峡药学》2009年第11期62-64,共3页Strait Pharmaceutical Journal

摘　　要：目的建立用高效液相色谱法测定复方银杏通脉胶囊中丹参酮ⅡA的含量。方法采用依利特C18色谱柱(250mm×4.6mm,5μm);甲醇-水(80∶20)为流动相;检测波长为270nm;流速:1.0mL·min-1。结果丹参酮ⅡA在69.04～780.80ng范围内与峰面积呈良好的线性关系,回归方程为Y=5.02210X-1.39888,(r=1)。丹参酮ⅡA的平均回收率为96.22%,RSD%为3.068%(n=5)。结论方法简便,准确,可靠,适用于复方银杏通脉胶囊的质量控制。OBJECTIVE To establish a method for determination of the content of tanshenone ⅡA in CO Yinxing Tongmai capsule by HPLC.METHODS The chromatographic conditions were：C18 column（250mm ×4.6 mm,5μm）;methanol-water（80∶20） as mobile phase;detection wavelength was 270nm; flow rate was 1.0mL·min^-1.RESULTS The linear range of tanshenone ⅡA was 69.04～780.80ng,the regression equation Y=5.02210X-1.39888（r=1）,the average recovery was 96.22% with RSD% of 3.068%（n=5）.CONCLUSION This method is simple,reliable,and suitable for the quality control of CO Yinxing Tongmai capsule.

关 键 词：高效液相色谱法 丹参酮ⅡA 含量测定 

分 类 号：R927.2[医药卫生—药学]