大鼠内毒素性肺损伤血清ACE活性变化与内皮细胞损伤的关系  

The Relation Between Changes of Serum ACE Activity and Pulmonary Vascular EC Damage in Lung Injury Induced by Endotoxin in Rats

作　　者：汪盛贤[1] 孟蓉[2] 潘传敬[1] 马建[3] 刘宽宇 钟萍 

机构地区：[1]成都军区总医院病理科,610083 [2]中国航空工业总公司成都363医院,610041 [3]成都军区机关第二门诊部,610015

出　　处：《中国微循环》1998年第3期150-153,共4页Journal of Chinese Microcirculation

摘　　要：应用比色法和电镜测定、观察了大鼠注射内毒素（L组）和氮芥＋内毒素（NL组）后6个时相点血清血管紧张素Ⅰ转换酶（ACE）的活性变化及肺血管内皮细胞（EC）的形态改变。结果表明：L组于注射内毒素后1、5和12h血清ACE显著高于对照组（P＜0．01），24h逐渐下降，至72h趋于正常；NL组于12、24hACE明显高于对照组（P＜0．05）。L组各时相均见EC不同程度的肿胀、退变，重者坏死；NL组EC病变明显减轻。两组血清ACE的动态变化均与肺EC超微结构的病变程度密切相关。提示检测血清ACE活性变化对判断急性肺损伤、EC病变程度和了解ACE在血液循环调节方面的作用有着重要的意义。The Changes of serum angiote ns in -converting en zyme (ACE ) activity were detected and theultrastructure of pulmonary vascular endothelial cells (EC) was observed at 1h, 5h, 12h, 24h,48h and 72h af-ter the injection of lipopolysaccharid (LPS,group L) or nitrogen mustard and LPS (group NL). The results showed that the serum ACE in group L was higher than that in the control group(P<0. 01) at 1h, 5h, 12h,24h and then decreased to the normal at 72h after LPS injection. At every intervaI,EC were swelling,degener-ating or necrotizing. In group NL,the serum ACE increased at 12h ahd 24h (P<0.05) after LPS injection. EC damage were markedly lighter than that in group L. The changes of serum ACE were related to the degrees of EC ultrastructure in both groups. These findings suggested that changes of ser7am ACE played an important role in judging the acute lung injury,the degrees of EC damage and the effect of serum ACE on the circula-tion.

关 键 词：肺损伤 内皮细胞 中性粒细胞 内毒素 ACE 

分 类 号：R563.02[医药卫生—呼吸系统]