玉龙雪山冰川雪坑中细菌多样性群落结构及其与气候环境的关系  

Bacterial diversity and community at Yulong Mountains and their relationship to climatic and environmental changes

作　　者：马晓军[1,2] 刘炜[2] 侯书贵[1] 陈拓[1] 秦大河[1] 

机构地区：[1]中国科学院寒区旱区环境与工程研究所冰冻圈科学国家重点实验室,兰州730000 [2]兰州大学生命科学学院,兰州730000

出　　处：《兰州大学学报（自然科学版）》2009年第6期94-100,共7页Journal of Lanzhou University(Natural Sciences)

基　　金：中国博士后科学基金项目(2005038173);中国科学院冰冻圈与环境联合重点实验室开放基金项目(2005-02);冰冻圈科学国家重点实验室自主课题(SKLCS-ZZ-2008-01)

摘　　要：通过DAPI荧光计数、恢复培养和变性梯度凝胶电泳,分析了玉龙雪山不同深度积雪中的细菌数量、多样性及其群落结构.结果显示:玉龙雪山雪坑样品中细菌数量和多样性指数与气候环境替代指标w(Ca^(2+)),w(Mg^(2+)),w(Cl^-)等具有相关性.可培养细菌隶属于三个不同系统发育群:Actinobacteria(37%),Firmicutes(11%)和Proteobacteria(α-Proteobacteria)(52%),其中α-Proteobacteria类为优势类群.DGGE测序结果表明细菌属β-Proteobacteria,Actinobacteria和Bacteroidetes类群,以β-Proteobacteria为优势类群,并且不同深度雪坑中细菌多样性和群落结构没有明显变化.结果表明:海洋型冰川雪中细菌数量和多样性、群落结构与大陆型冰川雪相比,冰雪微生物具有其特殊性.The total bacterial counts, bacterial diversity and community structure in mountain snow pits from the Mt. Yulong regions, China, were analyzed with 4,6-diamino-2-phenylindole （DAPI）, culture-dependent and culture-independent methods, denaturing gradient gel eleetrophoresis （DGGE）. The microbial abundance and diversity index have a close relationship with mineral particle concentration. These mineral particles, such as ω（Ca^2＋）, ω（Mg^2＋） and ω（Cl^-）etc, are good indicators of climate and environment. The 16S rRNA genes of cultured bacteria belong to following groups： Actinobacteria（37%）, Firmicutes（11%）, Proteobacteria（α-Proteobacteria） （52%）. Among these, α-Proteobacteria are dominant groups. DGGE band sequenced belongs to following groups：β-Proteobacteria, Aetinobacteria and Bacteroidetes. β-Proteobacteria are dominant groups. The bacterial diversity and community structures do not change obviously in different depth snow samples. Comparing the microbial abundance, diversity and community structures in snow of inland glacier and maritime glacier, the microorganism of maritime glacier has its particularity.

关 键 词：细菌多样性 变性梯度凝胶电泳 玉龙雪山 

分 类 号：Q938.11[生物学—微生物学]