矽肺结核分枝杆菌Km基因突变的临床研究  

Clinical study on Km gene mutation in mycobacterium tuberculosis strains isolated from pulmonary silicotuberculosis

作　　者：冯柏[1] 王国扬[1] 姜平[2] 李洪敏[3] 王巍[3] 张芳[1] 

机构地区：[1]北京京煤集团总医院尘肺结核科,北京102308 [2]北京门头沟上岸尘肺结核科 [3]解放军总医院第二附属医院结研所,北京100091

出　　处：《临床肺科杂志》2010年第1期24-25,共2页Journal of Clinical Pulmonary Medicine

摘　　要：目的分析25株矽肺结核分枝杆菌耐卡那霉素和Km基因突变情况。方法通过传统梯度药敏试验,聚合酶链反应-单链构象多态性(PCR-SSCP)和测序,分析临床耐药株Km基因的变化。结果矽肺结核药敏试验,卡那霉素耐药率为28%(7/25),25株临床分离株的16SrDNASSCP电泳图谱均与结核分枝杆菌标准相同,7株耐卡那霉素分离株中,2株29.6%(2/7)Km基因SSCP泳动异常。结论抗结核二线药中,卡那霉素已出现耐药,其部分耐药原因可能是由于分枝杆菌耐卡那霉素的Km基因突变所致。Objective To analyze kanamycin drug resistance and its Km gene mutation in 25 mycobacterium trbereulosis strains isolated from pulmonary silicotuberculosis. Methods All isolates were detected by traditional drug susceptibility test and all Km genes were detected by plymerase chain reaction-single strand conformation polymorphism（PCR-SSCP） and sequencing. Results We fonud that 7 isolates were resistant to kanamycin in 25 isolates. They had the same SSCP electrophpresis maps of 16S rDNA conpared with standard strain of mycobacterium tuberculosis. Moreover,2 isolates resistance to kanamycin showed different SSCP electrophoresis maps in 7 kanamycin resistant isolates. Conclusion The strains resistance to kanamycin have emerged. The Km gene mutation maybe contributes partially to kanamycin resistance in mycobacteria.

关 键 词：矽肺结核 卡那霉素 Km基因 耐药 

分 类 号：R521[医药卫生—内科学] R135.2[医药卫生—临床医学] R450